*Corresponding author: Mai S. Elsheikh, E-mail: mai.seoudy@yahoo.com, Tel.: +201001646806 (Received 29/03/2024, accepted 15/04/2024) DOI: 10.21608/EJVS.2024.280315.1974 ©2025 National Information and Documentation Center (NIDOC) A Comprehensive Analysis and Assessment of Vitamin D with Inflammatory Biomarkers Levels Among Acute COVID-19 Infected Patients Ahmed Gharib 1 , Nawal A. Hassanain 2 , Ahmed Maher 2 , Raafat M. Shaapan 2 , Abdelghany Aish 3 , Mohamed Elfeky 4 , Niveen M. Ghoraba 5 , Wafaa M. El zefzafy 6 , Samy Zaky 6 , Amin Abdel Baki 7 , Mahmoud H. Elsaied 8 , Mohamed S. Hegazy 6 and Mai S. Elsheikh 9⁕ 1 Department of Internal Medicine, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt. 2 Department of Zoonotic Diseases, Veterinary Research Institute, National Research Centre, 33 El-Bohouth St., Dokki, Giza, P.O. Box 12622, Egypt . 3 Hepatigastroentrology and Infectious Diseases, Shebien elkom Teaching Hospital. 4 Department of Gastroenterology and Hepatology, Cairo Fatimic hospital- Cairo, Egypt. 5 Department of Clinical Pathology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt. 6 Department of Hepatogastroenterology and Infectious Diseases, Faculty of Medicine Al -Azhar University, Cairo, Egypt. 7 Department Hepatology, Gastroenterology, and Infectious Diseases Institute, National Hepatology and Tropical Medicine Research Institute (NHTMRI). Cairo, Egypt. 8 Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt. 9 Department of Complementary Medicine, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt. Abstract HE coronavirus disease 2019 (COVID-19) pandemic is only one of several zoonotic illnesses that have caused epidemics over the years that have killed millions of people over the course of millennia. This study included 88 patients with acute COVID-19 infection from Egypt. They were classified in to 3 groups with mild, moderate, and severe COVID-19 manifestation. Patients subjected to full history taking and clinical examination, CT scans of the chest, PCR test for Covid19 and blood sampling for vitamin D and Comprehensive inflammatory markers analysis. The present study reported statistically significant increase of age, dyspnea and respiratory rate (p <0.05) in sever infected groups than mild or moderate groups of COVID-19 patients. Moreover, the primary analysis outcome was significantly associated mortality with reduced hemoglobin levels and elevated values of white blood cells, platelets, D-dimer, LDH, ferritin, blood urea and increased Prothrombin Time (p <0.05). However, insignificant statistical difference was found between the measured serum levels of Vitamin D in patients who recovered from those who died (96.58-± 38.34 vs 100.75 ± 80.62). This study concluded that COVID-19 severity correlates with multiple inflammatory biomarkers and coagulation factors. While the evidence concerning the therapeutic effect of vitamin D on the outcomes of acute respiratory infections is still controversial but a causal link between level of Vitamin D and the risk of COVID-19 severity and mortality not entirely excluded. Keywords: Vitamin D, COVID-19, Inflammatory markers, Patients, Mortality. T Egyptian Journal of Veterinary Sciences https://ejvs.journals.ekb.eg/ 39 Egypt. J. Vet. Sci. Vol. 56, No. 2, pp. 405-415 (2025)