Peptides 26 (2005) 647–652 A comparison of leptin and ghrelin levels in plasma and saliva of young healthy subjects  Suleyman Aydin a,∗ , ˙ Ihsan Halifeoglu a , ˙ Ibrahim H. Ozercan b , Fazilet Erman c , Nermin Kilic a , Suna Aydin d , Nevin ˙ Ilhan a , Necip ˙ Ilhan a , Yusuf Ozkan e , Nusret Akpolat b , Levent Sert b , Emrah Caylak a a Department of Biochemistry and Clinical Biochemistry, Firat University, Medical School (Firat Medical Center), 23119 Elazig, Turkey b Department of Pathology, Firat University, Medical School (Firat Medical Center), 23119 Elazig, Turkey c Health Sciences Vocational School, Firat University, Medical School (Firat Medical Center), 23119 Elazig, Turkey d Department of Medical Education, Firat University, Medical School (Firat Medical Center), 23119 Elazig, Turkey e Department of Endocrinology-Internal Medicine, Firat University, Medical School (Firat Medical Center), 23119 Elazig, Turkey Received 6 September 2004; received in revised form 5 November 2004; accepted 8 November 2004 Available online 15 December 2004 Abstract In the last 10 years, saliva has been increasingly used as a diagnostic fluid and in predictions of disease progression. Leptin and ghrelin are synthesized in several tissues including the salivary glands. The action of ghrelin is antagonistic to that of leptin. This study was undertaken to measure and compare the saliva ghrelin–leptin and plasma ghrelin–leptin levels in healthy young subjects. In 30 healthy subjects, after an overnight fast, saliva and plasma leptin levels were measured using the ELISA method while saliva and plasma immunoreactive ghrelin levels were measured using a commercial radioimmunoassay (RIA). The latter uses 125 I-labeled bioactive ghrelin as a tracer and a rabbit polyclonal antibody raised against full-length octanoylated human ghrelin (Phoenix, Europe, Karlsruhe, Germany). The results of this investigation revealed that saliva leptin levels (6.19 ± 2.10 g/l) were lower than plasma levels (7.39 ± 3.23 g/l) while saliva ghrelin levels (188.5 ± 84.7 pg/ml) were higher than plasma levels (126.4 ± 38.5 pg/ml), when male and female subjects were considered together. Saliva leptin levels (5.93 ± 1.94 g/l) were lower than plasma levels (6.22 ± 2.92 pg/ml) while saliva ghrelin levels (190.3 ± 80.2 pg/ml) were higher than plasma levels (120.4 ± 35.7 pg/ml) in young males. Saliva leptin levels (6.47 ± 2.29 g/l) were lower than plasma levels (8.73 ± 3.14 g/l) while saliva ghrelin levels (183.2 ± 90.2 pg/ml) were higher than plasma levels (129.3 ± 42.8 pg/ml) in young females, and both saliva and plasma leptin levels were slightly lower in male subjects in comparison with female subjects. Also, Immunohistochemistry study indicated that ghrelin positivity was found in ductus epithelium of salivary gland. We have demonstrated for the first time that saliva ghrelin levels were higher than in plasma while saliva leptin levels were almost the same as in plasma. Measurements of ghrelin and leptin in saliva is non-invasive, simple, and generally much preferred by patients and thus may be an acceptable alternative to plasma sampling. © 2004 Elsevier Inc. All rights reserved. Keywords: Ghrelin; Leptin; Saliva; Plasma; Immunohistochemistry  This work was presented as a poster at the 18th meeting of the Turkish Biochemical Societies held in Trabzon, Turkey, 15–19 May 2004. ∗ Corresponding author. Tel.: +90 424 233 3555/1609; fax: +90 424 236 8660. E-mail address: saydin1@hotmail.com (S. Aydin). 1. Introduction The use of saliva rather than blood for diagnosis has been increased as a diagnostic fluid in the last decade [38]. These include hormones (e.g. DHEA and cortisol), toxins (e.g. heavy metals, such as Pb and Cd) [25], various drugs and their metabolites (e.g. cotinine), measures of mucosal 0196-9781/$ – see front matter © 2004 Elsevier Inc. All rights reserved. doi:10.1016/j.peptides.2004.11.008