International Journal of Pharmaceutical and Medical Sciences ISSN 2319-1252 Original Research Article Available online at www.ijpms.com Vol. 1(2), August 2012 Page95 SIMULTANEOUS DETERMINATION OF RAMIPRIL, HYDROCHLOROTHIAZIDE AND TELMISARTAN BY RP-HPLC Shravan Bankey 1* , Shailendra Bindaiya 1 , Ritesh Patel 2 1. Rajeev Gandhi College of Pharmacy, Bhopal (M.P.), India 2. College of Pharmacy, IPS Academy, Indore (M.P.), India *Corresponding author mail id: bankey_shravan@yahoo.co.in Abstract A simple, accurate and precise RP-HPLC method has been developed for simultaneous determination of ramipril, hydrochlorothiazide and telmisartan in combined formulation (tablet). The chromatographic separation was achieved on a 250 x 4.6 mm, 5μ Inertsil C18 column. Eluents were monitored by absorbance at 218 and 270nm using a mixture of methanol: water (pH adjusted to 4.5 using dilute orthophosphoric acid) in the ratio of 72:28 (v/v) at a flow rate of 1.0 mL/min. The elution time for ramipril, hydrochlorothiazide and telmisartan were found 6.34, 4.55 and 7.39 min, respectively. Ramipril showed linearity in the range of 0.5-10 μg/ml at the wavelength 218nm with correlation coefficient (r 2 ) 0.9987. Hydrochlorothiazide and telmisartan showed linearity in the range of 2.5-25 μg/mland 4-40 μg/mL, respectively, at 270nm with correlation coefficient (r 2 ) 0.9992 and 0.9996, respectively. The proposed method was validated by testing its linearity, accuracy, precision, selectivity and LOD/LOQ values and it was successfully employed for the determination of ramipril, hydrochlorothiazide and telmisartan in pharmaceutical tablet formulations. Keyword: Ramipril, Hydrochlorothiazide, Telmisartan, simultaneous estimation, HPLC.