Research J. Pharm. and Tech.2 (2): April.-June. 2009 , 378 ISSN 0974-3618 www.rjptonline.org RESEARCH ARTICLE Analgesic, Anti–Inflammatory and Anti–Hemorrhoidal Activity of Aqueous Extract of Lantana Camara Linn BK Gidwani 1 * Sushil Bhargava 2 , SP Rao 2 , A Majoomdar 2 , DP Pawar 1, RN Alaspure 1 1 Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 4411100 2 Columbia institute of Pharmacy Tekari Raipur,493111 *Corresponding Author E-mail: bhargavasushil7@gmail.com ABSTRACT The aqueous extract of aerial parts of the plant Lantana camara was examined for the analgesic (hot plate method), anti–inflammatory (carrageenan induced paw oedema test) activities in albino rats by oral and topical routes respectively. The anti– hemorrhoidal activity was carried on patients using capsules prepared from dry aqueous extract of Lantana camara under the supervision of Chief medical officer, Department of shalya tantra Govt. Ayurvedic hospital, Nagpur. KEYWORDS: Lantana camara, Anti-inflammatory activity, Hot–plate test. INTRODUCTION: Lantana camara Linn. (Verbenaceae) commonly known as wild / red sage is the most widespread species of this genus. The aerial parts of many species of Lantana are widely used in folk remedies like cancer and tumors. A tea prepared from the leaves and flowers were taken against fever, influenza and stomachache. The species name, Lantana camara is probably adopted from the West Indian colloquial name for the common species. The other uses of plant show anti–malarial, anti–bacterial and anti–diarrhoeal activities. The present study was conducted to verify the analgesic, anti – inflammatory and anti–hemorrhoidal activity of aqueous extracts of Lantana camara. MATERIALS AND METHODS: Plant material: Aerial parts of the plant Lantana camara was collected and botanically identified at Department of Botany, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur. Voucher specimen has been deposited at the herbarium. Specimen Voucher No. is 9101 Preparation of Plant extract: The leaves of Lantana camara were air dried in shade, under normal environmental conditions and then subjected to size reduction to get coarse powder. Such powdered material was charged into the soxhlet Received on 22.01.2009 Modified on 16.04.2009 Accepted on 23.04.2009 © RJPT All right reserved Research J. Pharm. and Tech.2(2): April.-June.2009,;Page 378-381 apparatus, and successively extracted with organic solvents viz. petroleum ether, ethanol, acetone, methanol, hydro alcoholic and aqueous solvents. The aqueous and hydro alcoholic extractions were done by maceration process. 1 Further these extracts were subjected to phytochemical screening for the presence of active constituents and finally aqueous extract was used for the present work. Animals: The studies were carried out on albino rats (200 – 250g) of either sex. The animals were housed in group of five and maintained on a 12-h light: 12-h dark cycle with a standard pellet diet and clean drinking water ad libtum. Animal Ethical Committee approved the experimental protocol under guidelines of CPCSEA, New Delhi. Analgesic activity: Analgesic activity of aqueous extract of Lantana camara was screened by Eddy’s hot plate method. 2 Hot–plate test: The hot–plate test was performed following the method of Eddy and Leimbach with, modifications. Each rat was placed singly on the hot–plate at 56 ±1 0 C and the time for licking of the paws was recorded before, and 30 min after, the oral administration of the different extract solutions tested in two doses (300mg/kg and 500mg/kg), the vehicle (control group Propylene glycol 1ml/1oog) or the standard drug (Ibuprofen 100mg/kg). 3