International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.3, No.4, pp 1963-1970, Oct-Dec 2011 Synergic Activity of Fenugreek seeds and Neem leaf extracts against Alloxan induced diabetic rats. *Shravan Kumar Dholi 1 , Ramakrishna Raparla 2 , Kannappan 3 . 1 Depatment of Pharmacology, Vaageswari Institute of Pharmaceutical Sciences, Beside LMD Police Station, Ramakrishna Colony, Karimnagar, A.P.-505481, India. 2 Depatment of Pharmaceutics, Vaageswari Institute of Pharmaceutical Sciences, Beside LMD Police Station, Ramakrishna Colony, Karimnagar, A.P.-505481, India. 3 Department of Pharmacy, Annamali University, Annamali Nagar, Tamil Nadu-608002. India *Corres.author: shravan_21m@yahoo.com Mobile: + (91) 8008961562 Abstract: Diabetes is a metabolic disease in which a person has high blood sugar, either because the body does not produce enough insulin. Different studies have established the antidiabetic activity of fenugreek and neem. In present study our aim is to evaluate the antidiabetic effect of combined extracts in diabetic rats and find out the synergistic activity of both plant extracts if any. Glucose Tolerance Test suggested that efficacy of combined extract than their individual as the combined extract produced maximum fall of serum glucose levels 49.89% at 90 min after glucose administration. The Alloxan (120mg/kg .i.p.) used for induction of diabetes and Fenu Greek seed extract and Neem leaf extract used at a dose of 100mg/kg and 100mg/kg represents for single extract treatment, where as FSE and NLE 50mg/kg, 50mg/kg in combined extracts treatment for 28 days study. Glibenclamide used as standard drug. After 28 treatment the extracts significantly reduces serum glucose levels, the combined treatment found most effective. Combined extracts produces 52.45%, 20.45%, 24.58%,25.1%,24.6% reduction in serum cholesterol, triglycerides,LDLs, Creatinine.Urea respectively, increased 25.28% HDL levels after 28 days treatment which is better than the individual effect of extract. The results suggested the synergistic antidiabetic activity of MEFS and EENL when administered together. Key words: Diabetes, glucose, Fenu Greek, Neem leaf Cholesterol, Urea, Creatinine, Triglycerides. INTRODUCTION Diabetes is a metabolic disease in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is produced. This high blood sugar produces the classical symptoms of Polyuria (frequent urination), polydipsia (increased thirst) and polyphagia (increased hunger). Based on the WHO recommendations hypoglycemic agents of plant origin used in traditional medicines are important 1 . Plant drugs and formulations are frequently considered to be less toxic and more free side effects than synthetic one 2 .There are numerous traditional medicinal plants reported to have hypoglycemic properties such as Allium sativum (Garlic), Azadirachta indica (Neem),