52 Scientific Journal of Biological Sciences (2012) 1(2) 52-60 Heamolytic activities, deoxyribonuclease production and in-vitro flouroquinolones susceptibility profile of aerobic gram positive cocci associated with acne vulgaris O.J. Akinjogunla*, O.I. Medo, E.P. Philip Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria * Corresponding author; Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria A R T I C L E I N F O Article history: Received 08 August 2012 Accepted 25 August 2012 Available online 29 August 2012 Keywords: Acne vulgaris Fluoroquinolones Deoxyribonuclease Heamolysis Susceptibility A B S T R A C T Bacteriological investigations were carried out on forty–six (46) swabbed samples from the pustular and nodulocystic skin lesions of undergraduate students with acne vulgaris using standard bacteriological methods. The fluoroquinolones susceptibility profiles, heamolytic activities, deoxyribonuclease production were determined using Kirby –Bauer disc diffusion methods, Columbia Blood Agar (CBA) and Deoxyribonuclease (DNase) agar, respectively. The results showed that all the 46 clinical samples showed positive growth, with 24 (52.2%) having single bacterium isolated. Co- infection with two bacterial species was seen in 24 (28.3%), while polybacterial growth was present in 9 (19.6%) of the samples. Staphylococcus aureus was the most prevalent aerobic Gram positive cocci associated with acne vulgaris with 32 (42.1%) occurrences, followed by Staphylococcus epidermidis 26 (34.2%) and MiĐƌoĐoĐĐus spp ϭϴ ;Ϯϯ.ϵ%Ϳ. “idžteeŶ of the isolates pƌoduĐed α – haeŵolLJsiŶ, Ϯϱ ;ϯϮ.ϵ%Ϳ pƌoduĐed β – haemolysin and 35 (46.1%) produĐed Ŷo haeŵolLJsiŶ ;γ-haeŵolLJsisͿ. Of the ϭϲ α – haemolysin producers, Staphylococcus aureus produced the highest 9 (56.3%), followed by Staphylococcus epidermidis 5 (31.3%) and Micrococcus spp 2(12.5%). Of the seventy – six aerobic Gram positive cocci isolated only 33 (43.4) were DNase producers, while 43 (56.6) were non-DNase producers. Staphylococcus aureus were highly sensitive to Moxifloxacin, while 26 (81.3%) were sensitive to Lomefloxacin and Gatifloxacin. Staphylococcus epidermidis and Contents lists available at Sjournals Journal homepage: www.Sjournals.com Original article