S861 Document heading doi: 襆2012 by the Asian Pacific Journal of Tropical Disease. All rights reserved. Evaluation of Chitinase producing and antimicrobial properties of streptomyces isolated from shrimp shell disposable area. Subramanian Kumaran * Balaraman Deivasigamani * , Uttara Vairagkar, Sadaiappan Balamurugan & Manikkam Sakthivel CAS in Marine Biology Faculty of Marine Sciences Annamalai University Parangipettai Tamil Nadu, India Asian Pacific Journal of Tropical Disease (2012)S861-S864 Asian Pacific Journal of Tropical Disease journal homepage:www.elsevier.com/locate/apjtd *Corresponding author: Contact information CAS in Marine Biology Faculty of Marine Sciences Annamalai University Parangipettai - 608502 Tamil Nadu India Tel : +91-9943215332; +91-9443880023 E-mail:kumarun23@gmail.com; b.deivasigamani@gmail.com 1. Introduction Chitin is one of the most abundant natural polysaccharides in the world. Global production of crustacean shellfish has been estimated to be 1. 9 million metric tonnes [1] . During processing of shrimps its head, shell and tail portionsn are removed which generates 1.44 million metric tonnes of protein and chitin rich substances every year [2] . Thus, attention must be paid to greater utilization of shrimp processing by-products in order to address such concerns. Shrimp waste contains protein and chitin in range 33-40 and 17-20% on dry weight basis, respectively and hence can serve as a cheap substrate for microbial protease and chitinase production [3] . Chitinases, a group of enzymes capable of degrading chitin directly to low- molecular-weight products, have been shown to be produced by a number of microorganisms. Almost all of the reported chitinase-producing strains will use chitin or colloidal chitin as a carbon source. The production of inexpensive chitinolytic enzymes is an important element in the utilization of shellfish wastes that not only solves environmental problems but also promotes the economic value of the marine products [4] . Streptomycetes are soil-dwelling mycelial bacteria that produce a large number of secreted proteins and many secondary metabolites, including important antibiotics. Chitin is a major nutrient source for many streptomycetes, and these microorganisms have developed complex extracellular systems for chitin utilization [5] . Among the various industrially important microorganisms, actinobacteria are of prime importance and are primarily recognizes as organisms of academic curiosity and also as potential antibiotic producers. Actinobacteria are the ARTICLE INFO ABSTRACT Article history: Received 15 September 2012 Received in revised from 27 October 2012 Accepted 28 November 2012 Available online 28 December 2012 Keywords: Chitinase Degradation Antibacterial activity Streptomyces Objective: At present scenario need the potential medical application enzymes and control the major clinical bacterial pathogens produce infections in humans. In this mind isolate and characterization of chitinase and antibacterial compounds from streptomyces. Methods: The actinobacterial strains isolated from crab and shrimp shells disposable area (Parangipettai) were screened for chitinolytic activity on colloidal chitin-agar plates. After incubation the clear zone producing strain were selected for chitinase production. Every 2 days interval chitinolytic activity was measured and protein estimation was determined. Antagonistic activity of chitinase producing actinobacterial isolates were tested by adopting agar plug method. Based on the preliminary screening results, chitinase producing strain was used for bioactive substance production through submerged fermentation by adopting shake flask method. Antibacterial activity of crude extracts was tested by disc diffusion method at 100毺g/disc concentration. Results: totally 15 actinobacterial strain were isolated. Based on the zone formation on chitin minimal plate CDB20 showed promising zone activity. The CDB20 strain was inoculated in chitin production medium at 10 days. After incubation the chitinase activity showed 1.22U/ml protein estimation 14mg/ ml. The preliminary screening showed promising antibacterial activity against clinical bacterial pathogens. Secondary screening results showed maximum K. pneumonia(23mm) and minimum S. aureus (18mm) was observed. The potent strain identified as genera streptomyces. Conclusion: The isolated potent streptomyces have degraded chitin and inhibited the clinical bacterial pathogens. In future in this strain will be used for waste shrimp and crab shells management and recycling and In this strain produce chitinase and antibacterial compounds have more medical application. So this strain has multi functional applications. Contents lists available at ScienceDirect