European Journal of Biological Sciences 5 (1): 09-13, 2013 ISSN 2079-2085 © IDOSI Publications, 2013 DOI: 10.5829/idosi.ejbs.2013.5.1.7292 Corresponding Author: Anuraj Nayarisseri, In silico Research Laboratory, Eminent Biosciences, Indore, India-452010. Tel: +91 9752295342; E-mail-anuraj@eminentbio.com. 9 Screening, Isolation and Identification of Polygalacturonase Producing Bacillus tequilensis Strain EMBS083 Using 16S rRNA Gene Sequencing K. Pratik Shah, K. Harshpreet Chandok, Pragya Rathore, 1 1,2 3 M. Varsha Sharma, Mukesh Yadav and S. Anuraj Nayarisseri 4 1,2 1 In silico Research Laboratory, Eminent Biosciences, Indore, India-452010 1 Department of Biotechnology, Softvision College, Indore, India-452010 2 Department of Biotechnology, Sanghvi Institute of Management and Science, Indore-453 331 3 Department of Biotechnology, Banasthali University, Rajasthan, India-304022 4 Abstract: Polygalacturonase is depolymerizing enzymes which are produced in plants and are foremost for industrial applications such as pretreatment of waste water from vegetable food processing industries. Mostly pectinaceous containing material are produced by bacteria and fungi. Therefore, in the present study, We aimed to identify a novel strain of Polygalacturonase producing Bacillus tequilensis. The sample was isolated from soil and rotten vegetables found in the geographical location of Indore, Madhyapradesh, India. The same has been serially diluted and were subjected to 16S rDNA sequencing. The sequence obtained from the isolate has been followed for a pair wise alignment using BLAST against other species of Bacillus tequilensis which constitute to be a novel species of the same, which was named further as Bacillus tequilensis sp. EMBS083. After the characterization the sequence of isolate has been deposited in GenBank with accession number KC171015. Key words: Bacillus tequilensis %Bacillus tequilensis Sp. EMBS083 %Polygalacturonase Producing Bacteria %16S rRNA Gene Sequencing INTRODUCTION production of papers and bast fibres, natural fibers Pectinases are a heterogeneous group of enzymes [3, 5, 6]. Bacillus tequilensis is a Gram-positive, 0.9x4.0 that hydrolyze the pectic substances present in plants. μm, single cell, motile rod shape bacteria. Biochemically Pectinase is an enzyme group that catalyzes pectic Bacillus tequilensis is quite similar to Bacillus substance by degradation reactions through tequilensis which can be differentiated by positive depolymerization (hydrolases and lyases) and de- arginine dihydrolase, lysine decarboxylase, ornithine esterification (esterases) reactions. These polysaccharide decarboxylase and acid production from rhamnose [7]. degrading enzymes are suitable tools to study the This study was concerned with the searching of structure of pectin [1]. Polygalacturonase is of prime novel bacterial cultures from different habitats for the importance for plants since they assist in cell-wall large scale commercial production of polygalacturonases. extension and softening of some plant tissues during This effort can fulfill the national demand and strengthen maturation and storage [2, 3]. Pectinases are most widely the industry and economy because valuable foreign distributed enzymes in bacteria, fungi and plants [4]. exchange is spent on import of industrial enzymes. The pectinolytic enzymes degrade the pectic substances Therefore in the present study, we describe the isolation found in plant tissues, thereby, having numerous of a novel strain of pectinase producing Bacillus applications in various types of industries such as tequilensis. treatment in textile industries, paper and pulp Industry