1 RUMEN FERMENTATION OF BALI CATTLE FED AGRICULTURE WASTE PRODUCT FERMENTED BY RUMEN LIQUOR by: Mudita, I M., T.I. Putri, T.G.B. Yadnya, I G. L. O. Cakra, and Budi Rahayu T. P Faculty of Animal Husbandry Udayana University HP. 081338791005 email: muditafapet_unud@yahoo.com ABSTRACT This research had been carried out to increase the rumen fermentation of Bali cattle fed ration non-conventional waste through fermentation of the rumen liquor. Randomized Block Designed with four treatments and three blocks were used in this experiment. The first treatment was ration non-conventional waste without fermentation (RL 0 ), while the other three treatments were RL 0 fermented by 20 ml, 40 ml and 60 ml rumen liquor/kg ration respectively as a treatments (RL 20 , RL 40 and RL 60 ). The result showed that fermentation of the rumen liquor were significantly increased (P<0.05) degraded of dry matter in the rumen/DDMR (9.05–15.58%), degraded of organic matter in the rumen/DOMR (11.19–11.84%), degraded of crude protein in the rumen/DCPR (4.08–7.88%), and degraded of gross energy in the rumen/DGER (20.09–25.53%) of Bali cattle compared than RL 0 , but was not significantly increased (P>0.05) of degraded of crude fiber in the rumen/DCFR. Production of total VFA, acetate acid and propionate acid were significantly higher (P<0.05) are 26.89–84.73%, 32.25–77.25% and 58.96– 181.00% respectively compared than RL 0 , Even though the amount protozoa, pH rumen, NH 3 -N, butyrate acid concentration, purine absorbed, microbial N synthesis, and microbial protein synthesis in the rumen were similar (P>0.05) in all treatments. It was concluded that fermentation of 60 ml rumen liquor/kg ration produce highest the degradable of dry matter and organic matter in the rumen, total VFA and propionic acids. Key words: Bali cattle, Fermentation, Non-Conventional Waste, Rumen Liquor INTRODUCTION Increasing the rumen fermentation on degrading fibrous feed such as non- conventional feedstuffs is one of Bali cattle productivity improvement strategy. The products of rumen fermentation such as degraded nutrients in the rumen, metabolic rumen product (VFA, and NH 3 -N) and microbial biomass are essential nutrients to meet the nutrient demands of the anaerobic microbes and body tissues of ruminant animals (Mohamed and Chaudhry, 2008). The degradable nutrients like as polimer and monomer carbohidrates, N compounds (amino acid, peptida and polipeptida) are source nutrients for activities of the rumen microbes and the host animal. The microbial fermentation products eventually become available as energy (volatile fatty acids/VFA) and protein (microbial biomass) for animal tissue metabolism. Exactly N compounds