Vol. 2(4) Oct - Dec 2011 www.ijrpbsonline.com 1663 International Journal of Research in Pharmaceutical and Biomedical Sciences ISSN: 2229-3701 __________________________________________Research Paper Estimation of Prednisolone in Proliposomal formulation using RP HPLC method. Mallesh kurakula 1 , Abdul Bari Mohd 1 . , Samhuidrom 2 , Padmanabha Rao A 2 , Prakash V Diwan* 1 Department of Pharmaceutics, 2 Department of Pharmaceutical analysis, School of pharmacy (Formerly Lalitha college of Pharmacy), Venkatapur (V), Ghatkesar (M), R.R district, Andhra Pradesh, INDIA. ABSTRACT The present study was aimed to develop a newer analytical method for determination of prednisolone in proliposome formulation. The RP-HPLCmet hod was developed using phenomenex Luna C18 analytical column comprising 5μm particles with 4.6×250mm of column dimensions. The mobile phase comprising of acetonitrile: 0.025M potassium dihydrogen ortho phosphate buffer [pH-3.0] in the ratio (60:40) v/ v. The flow rate was maintained at 1.0ml/ min and elut e was monit ored by using U.V det ector at 244nm.The retention time of prednisolone was 6.4 minut es. The method was validated for its specificit y, accuracy, precision, and linearity, limit of detection, limit of quantification, robustness and stability parameters. LOD and LOQ were 35 and 62.5ng/ ml respectively. The linearity of the drug was in the range of 1-5μg/ ml with co-efficient of correlation 0.999. The percent recovery of t he prednisolone drug was 100.64%. The proposed met hod can be used for quantitative and entrapment efficiency determination of prednisolone in proliposome formulation. Key words : Prednisolone, Anti-Inflammatory Drug, proliposome formulation, RP- HPLC met hod, Stability, Rheumatoid arthritis. INTRODUCTION Prednisolone (11β)-11,17,21-trihydroxypregna-1,4- diene-3,20-dione [fig.1] is an steroidal drug with predominant glucocorticoid and low mineral corticoid activity. It is mainly used for the treatment of a wide range of inflammatory and auto-immune diseases[1] such as asthma[2], multiple sclerosis[3], rheumatoid arthritis[4], autoimmune hepatitis[5] etc. Prednisolone is also known as ‘disease modifying anti-arthritic drugs’ because of its anti inflammatory [6] action by inhibiting gene transcription for COX-2, cytokines, cell adhesion molecules, and inducible NO synthetase [7]. The main objective of the study is to develop and validate HPLC method so as to obtain an accurate, sensitive and precise for quantitative determination of prednisolone in proliposomal formulation. Detailed literature survey revealed that the available analytical methods for prednisolone alone or in combination with other drugs is reported by using UV Spectrophotometry [8], RP- HPLC [9], and matrix dispersion liquid _______________________________________ *Address for correspondence: E-mail: diwanpv@gmail.com chromatography [10]. But no methods have been reported for the determination of prednisolone in proliposome formulation. The present work is aimed at determining Prednisolone in Proliposomal formulation using RP-HPLC. The attempts also made for determining the stability of the formulation prepared under forced degradation conditions. MATERIALS AND METHODS Chemical: Prednisolone (API) was a gift sample from AKUMS Laboratories Ltd., Haridwar. Acetonitrile, potassium dihydrogen ortho phosphate and ortho phosphoric acid used in the study were of analytical grade and obtained from Qualigens Ltd., Mumbai. HPLC grade water was obtained from SD-Labostar (3 TWF-UV) water purification system. The chromatographic condition for method was optimized [12] and depicted in Table1.