World Applied Sciences Journal 20 (4): 540-544, 2012 ISSN 1818-4952 © IDOSI Publications, 2012 DOI: 10.5829/idosi.wasj.2012.20.04.1718 Corresponding Author: Abdur Rauf, Institute of Chemical Sciences, University of Peshawar, Peshawar, KPK, Pakistan. Tel: +92-346-9488944/313-9488944. 540 Antibacterial and Phytotoxic Profile of Selected Pakistani Medicinal Plants Abdur Rauf, Naveed Muhammad, Ajmal Khan, 1 2 3 Nizam Uddin, Muhammad Atif and Barkatullah 3,4 1 5 Institute of Chemical Sciences, University of Peshawar, Peshawar -25120, KPK, Pakistan 1 Department of Pharmacy University of Peshawar, Peshawar, Pakistan 2 H.E.J. Research Institute of Chemistry, International Center of Chemical and 3 Biological Sciences, University of Karachi, Karachi-75270, Pakistan Center of Excellence in Marine Biology, University of Karachi, Karachi-75270, Pakistan 4 Department of Botany University of Peshawar, Peshawar, Pakistan 5 Abstract: In the present research work three Pakistani medicinal plants were scrutinized for their antibacterial and phytotoxic potentials. The antibacterial and phytotoxic effects were determined for the crude methanolic extract and its subsequent solvent fractions. In case of antibacterial effect the chloroform and ethyl acetate fractions of all tested plants showed moderate antibacterial potential. All the tested plants were proved good phytotoxic at concentrations of 1000, 100 and 10 ppm. The phytotoxic effect of the tested plants was higher than antibacterial effect. In conclusion these plants can be used as good source of antibacterial and phytotoxic actions. Key words: Periploca aphylla %Ficus sarmentosa %Isodon rugosus %Phytotoxic activity %Antibacterial activity INTRODUCATION indigenous climber and its leaves are lopped for feeding Plants are natural source of producing wide number segments occupying the steep terrain [4]. Isodon rugosus of bioactive chemical constituents in a most efficient belong to family Lamiaceae. Is a deciduous shrub way and with precise selectivity. Since the middle of the growing up to 1.5 m. It flowers from July to September and 19 century, different class of bioactive compounds have the seeds ripen from August to October. It has suitable th been isolated and characterized. Many of these are used for light (sandy), medium (loamy) and heavy (clay) soils. as the active ingredients of the modern medicine, or as the The suitable pH for this plant is acid, neutral and lead compounds for new drugs discovery. Several plant basic (alkaline) soils. It can propagate in semi-shade derived medicines, are rich in phenolic compounds such (light woodland) or no shade and prefers moist soil. , as those used in protection against coronary heart One report mentioned that the plant is edible, but no data diseases and carcinogenesis [1-2]. are reported to show which part of the plant is used [5]. Periploca aphylla belongs to family Asclepiadaceae, In current study three medicinal plants were evaluated for is a large erect branched shrub, grown on different their antibacterial and phytotoxic activities. altitudes, all over South West Asia. P. aphylla is commonly used for the treatment of tumours and MATERIALS AND METHODS swellings, while the bark of the P. aphylla used as antipyretic [3]. Ficus sarmentosa is commonly found at N. Plant Material: Periploca aphylla, Ficus sarmentosa W. Hills, Kashmir, Northern India, Bangla Desh, Burma and Isodon rugosus were collected from Razagram, and China. It is extremely variable and common wild Toormang, Dir, Khyber Pakhtunkhwa Pakistan in month species. The plant is found creeping on rocks and in of December 2011. The plants were identified by Ghulam crevices or climbing on other trees with the help of Jelani Department of Botany University of Peshawar adventitious roots, up to c. 2300 m from sea level. It is an Pakistan. all types of animals particularly by the marginal social