Journal of Prosthodontics Vol. 4 No.1 Jan –Jun 2013 :22-26 22 Efek Seduhan Bunga Rosella dalam Menghambat Pertumbuhan Candida albicans Pada Resin Akrilik (The Effect of Roselle Flower Infusion in Inhibiting Candida albicans Colonization on Acrylic Resin) Ashri Ratnasari 1 , Wahjuni Widajati 2 , Nike Hendrijantini 2 1 Mahasiswa Program Studi Pendidikan Dokter Gigi Fakultas Kedokteran Gigi Universitas Airlangga 2 Departemen Prostodonsia Fakultas Kedokteran Gigi Universitas Airlangga Surabaya – Indonesia ABSTRACT Background: Acrylic resin has been commonly used as denture base. As denture base, acrylic resin has tendency to be attached by Candida albicans. To prevent over colonization of Candida albicans, acrylic resin must be cleaned properly once a day. Besides, fabricated denture cleanser is known containing allergen. It is needed to find an alternative antifungal agent. Some experiments have proved that flavonoid has antifungal effect. Roselle flower is known containing flavonoid. Purpose: This study has purpose to find out the effect of roselle flower infusion and to find out its effective concentration in inhibiting Candida albicans colonization on acrylic resin. Material and method: Twenty eight samples suitable with requirements were exposed with saliva and Candida albicans suspension. These samples were divided into 4 groups and then each group is soaked in sterile aquadest, roselle flower infusion 20%, roselle flower infusion 30%, and roselle flower infusion 40%. They were incubated for 48 hours and the Candida albicans colonies then were counted. Data obtained were analyzed using Kolmogorov-Smirnov Test, Levene’s Test, One-Way Anova Test, and Least Significance Difference Test. Results: All groups soaked in roselle flower infusion showed decreasing Candida albicans colonization. Statistically, there is significant difference between each group, p value = 0,00 (p<0,05). Conclusion: Roselle flower infusion has effect to inhibit Candida albicans colonization in acrylic resin. The effective concentration of roselle flower infusion to inhibit Candida albicans colonization is 30%. Key words: Candida albicans, roselle flower infusion, acrylic resin Korespondensi (correspondency): Ashri Ratnasari. Fakultas Kedokteran Gigi Universitas Airlangga. Jl. Mayjen. Prof. Dr. Moestopo No. 47 Surabaya 60131, Indonesia. Email: ashri_020911143@yahoo.com. PENDAHULUAN Resin akrilik merupakan bahan yang umum digunakan sebagai basis gigi tiruan. Sebagai basis gigi tiruan, resin akrilik merupakan bagian gigi tiruan yang paling banyak bersentuhan dengan mukosa rongga mulut. 1,2 Penggunaan gigi tiruan dalam waktu lama dapat menghalangi proses pembersihan permukaan mukosa rongga mulut oleh lidah dan saliva. Hal ini mengakibatkan mudah terjadinya perlekatan Candida albicans pada basis akrilik. Perlekatan Candida albicans merupakan tahap awal kolonisasi Candida albicans. Jumlah koloni Candida yang berlebihan dalam rongga mulut dapat menyebabkan terjadinya oral candidiasis. 3 Desinfeksi pada gigi tiruan diperlukan untuk membersihkan gigi tiruan dari koloni Candida albicans yang berlebihan. 4 Research Report