1 3 Theor Appl Genet (2013) 126:2451–2465 DOI 10.1007/s00122-013-2146-1 ORIGINAL PAPER Increasing the density of markers around a major QTL controlling resistance to angular leaf spot in common bean Paula Rodrigues Oblessuc · Juliana Morini Kupper Cardoso Perseguini · Renata Moro Baroni · Alisson Fernando Chiorato · Sérgio Augusto Morais Carbonell · Jorge Mauricio Costa Mondego · Ramon Oliveira Vidal · Luis Eduardo Aranha Camargo · Luciana Lasry Benchimol-Reis Received: 1 February 2013 / Accepted: 14 June 2013 / Published online: 6 July 2013 © Springer-Verlag Berlin Heidelberg 2013 3.0 cM. The position of the SSR ATA220 coincided with the maximum LOD score of the QTL. Moreover, a new QTL (ALS10.2 UC ) was identified at the end of the same link- age group. Sequence analysis using the P. vulgaris genome located ten SSRs and seven STS-DArT on chromosome 10 (Pv10). Coincident linkage and genome positions of five markers enabled the definition of a core region for ALS10.1 spanning 5.3 Mb. These markers are linked to putative genes related to disease resistance such as glycosyl trans- ferase, ankyrin repeat-containing, phospholipase, and squa- mosa-promoter binding protein. Synteny analysis between ALS10.1 markers and the genome of soybean suggested a dynamic evolution of this locus in the common bean. The present study resulted in the identification of new candidate genes and markers closely linked to a major ALS disease resistance QTL, which can be used in marker-assisted selec- tion, fine mapping and positional QTL cloning. Abstract Angular leaf spot (ALS) causes major yield losses in the common bean (Phaseolus vulgaris L.), an important protein source in the human diet. This study describes the saturation around a major quantitative trait locus (QTL) region, ALS10.1, controlling resistance to ALS located on linkage group Pv10 and explores the genomic context of this region using available data from the P. vul- garis genome sequence. DArT-derived markers (STS-DArT) selected by bulk segregant analysis and SCAR and SSR markers were used to increase the resolution of the QTL, reducing the confidence interval of ALS10.1 from 13.4 to Communicated by B. Diers. Electronic supplementary material The online version of this article (doi:10.1007/s00122-013-2146-1) contains supplementary material, which is available to authorized users. P. R. Oblessuc (*) · J. M. K. Cardoso Perseguini · R. M. Baroni Departamento de Genética e Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo 13083-970, Brazil e-mail: parobl@gmail.com J. M. K. Cardoso Perseguini e-mail: jukupper@gmail.com R. M. Baroni e-mail: renatabaroni19@gmail.com P. R. Oblessuc · J. M. K. Cardoso Perseguini · R. M. Baroni · J. M. C. Mondego · L. L. Benchimol-Reis Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, São Paulo 13001-970, Brazil J. M. C. Mondego e-mail: jmcmondego@iac.sp.gov.br L. L. Benchimol-Reis e-mail: llasry@iac.sp.gov.br A. F. Chiorato · S. A. M. Carbonell Centro de Grãos e Fibras, Instituto Agronômico de Campinas (IAC), Campinas, São Paulo 13001-970, Brazil e-mail: afchiorato@iac.sp.gov.br S. A. M. Carbonell e-mail: carbonel@iac.sp.gov.br R. O. Vidal CHU Sainte-Justine Research Center, Université de Montréal, Montréal, QC, Canada e-mail: ramon.vidal@gmail.com L. E. A. Camargo Departamento de Fitopatologia e Nematologia, Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz (USP-ESALQ), Piracicaba, São Paulo 13418-900, Brazil e-mail: leacamar@usp.br