International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.5, No.4, pp 1428-1436, April-June 2013 Bioprocessing Of Ar I solates For Economical Production Of L – Glutaminase By Solid State Fermentation S. Aravinth Vijay Jesuraj* 1 , S. Marylin Jeya Praya 2 , R. Bino Kingsley 2 , L. Dinesh Kumar 2 , M. Ravikumar 3 1 JNT University, Kukatpally, Hyderabad, 500 085, Andhra Pradesh, India. 2 Allianze University College of Medical Sciences, Pulau Pinang, 13200, Malaysia. 3 Geethanjali College of Pharmacy, Cheerial, Keesara, RR dist. Andrapradesh, India. *Corres.author: vijay2nov@gmail.com Abstract: L-glutaminase (L- glutamine amidohydrolase EC, 3.5.1.2) is a significant enzyme found to possess antileukaemic properties and also having many other applications. The main objective of the present work was to produce potential strains in producing the enzyme with agro-industrial wastes such as Decaffeinated Tea Dust Waste (DTDW) and Coconut Fiber Waste (CFW) which were fortified with whey. Rainforest soil samples from different places were collected for potential strains and were introduced into selective medium where L- glutamine was the only carbon and nitrogen source. Such grown organisms were screened by rapid plate assay. From the total 16 strains 3 prominent strains (AR-glut 5, AR-glut 6, AR-glut 7) were selected from its zone of colour formation. Further they were identified through different biochemical tests of Bergey’s manual of bacterial classification. Organisms were optimized to yield more enzyme for their medium pH, incubation temperature and inoculum volume. The effect of additional phosphate and metal ion sources were also studied. Enzyme assay was carried out by nesslerization and analyzed using spectrophotometer at 450 nm. Enzyme activity was calculated from the standard graph of ammonium sulphate. The optimized temperature, pH, inoculum volume, and incubation were found to get a maximum yield of enzyme at 37 0 C, 7.4 pH, 15 mL and 48 hours respectively. Addition of Phosphate and metal ions were found to improve the yield of enzyme slightly. At the optimum pH, temperature, inoculum volume and incubation time the maximum yield of the enzyme was 150 IU/gds with AR-glut 7 on DTDW medium. DTDW and CFW prove to be wonderful materials in producing the enzyme L-glutaminase economically. Under optimized conditions the DTDW was found to be superior to that of CFW in enzyme production. Keywords: Solid state fermentation, L- glutaminase, Decaffeinated Tea Dust Waste medium, Coconut Fiber Waste medium, AR isolates. Introduction and Experimental Introduction: L-Glutaminase (L- glutamine amidohydrolase EC, 3.5.1.2) is an enzyme which catalyses deamidation of L- glutamine to L-glutamic acid and ammonia. It plays a significant role in cellular nitrogen metabolism. It is found to be a potent antileukaemic agent by reducing the glutamine pool, which is being avidly consumed by the tumor than the normal cells and there by destroying tumor cells selectively 2-5 . Some studies suggest it has a promising effect against retro viruses 1, 5 . It is also being used as analytical agent 11 and a bio-sensing agent in the determination of glutamine of biological products 10 . With all these potential properties