N Save Nature to Survive 8(2): 603-609, 2013 (Supplement on Medicinal plants) www.thebioscan.in 603 HPTLC FINGER PRINT ANALYSIS OF PHYTOPHENOLS OF PAEDERIA FOETIDA UNDER DIFFERENT EXTRACTION REGIMEN M. R. SENAPATI, P. C. BEHERA*, P. C. BISOI, A. MAITY AND S. C. PARIJA Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubanewswar - 753 003, Odisha, INDIA e-mail: drbeheraovc@gmail.com INTRODUCTION The scientific world, in recent era of medicine emphasizes on phyto-constituents as lead molecules in development of future generation safe drugs. This is because, large diversity of phytophnols and flavonoids lack adverse effects and possess free radical scavenging activity to reduce risks of cancer, cardiovascular disease, diabetes, inflammation, bacterial disease and ageing related diseases (Kumar and Surh, 2008). Phyto-constituents vary in chemical characteristics, polarities and distribution in the plant matrix (Sultana et al., 2009) and their recovery depends on extraction techniques and the nature/ volume of solvent (Turkmen et al., 2006). Conventional extraction techniques with water as solvent recover less constituent and is time consuming. On the other hand, advanced technique of microwave assisted extraction with polar solvents consumes less time and solvent and claims to extract even thermo-labile constituents also (Mandal et al., 2007). The qualitative and quantitative analysis of phyto- constituents by different methods exhibits the efficacy of the technique and the solvent of extraction (Behera et al., 2012b). High Pressure Thin Layer Chromatography (HPTLC) finger print is a step ahead of such analysis with constitutional details. So, screening of these techniques and solvents on recovery of different phyto-constituents by HPTLC finger print is more suitable than the traditional qualitative and quantitative estimation (Behera et al., 2012a). Water being non-polar solvent fails to extract all active principles and thereby potency of the phyto-constituent suffers to be suppressed. Sometimes, conventional extraction method with water is equally responsible for low potency of herbal formulations. The plants may have some more components to be extracted for better performance. So, there is a need to compare the constituents recovered in different methods and solvents for potency augmentation of phyto-medicines. Paederia foetida (shunk vine) of Rubiaceae family is an aromatic perennial climbing shrub. It is native to eastern Asia and grown in grassy hillsides, forests, river banks, waste grounds, roadsides on fences and urban areas of Assam, Bihar, Bengal and Odisha. It is a well known medicinal shrub for its antibacterial (Senapati et al., 2013a), Cytotoxic (Morshed et al ., 2012), anthelmintic (Dey and Pal, 2011), anti- hyperglycemic (Khan et al., 2011), hepato-protective (Uddin et al., 2011), anti-fungal (Majumdar et al., 2011), anti-ulcer (Reddy et al., 2011), antioxidative (Osman et al., 2009) and anti-diarrhoeal (Afroz et al., 2006) activities in several countries. Indian tribes of Orissa province use aqueous paste of this shrub traditionally for treatment of rheumatoid arthritis, hepatic disorders, piles, diabetes, asthma, coughs, body ache, itches, wounds, stomach-ache, diarrhoea, dysentery, flatulency and toothache. Therefore, the present study aims to screen the phenolic components of Paederia foetida shoot extracted in different groups of solvents following the methods of Cold Percolation (CP) and Microwave Assisted Extraction (MAE) through qualitative, quantitative and HPTLC finger print analysis with an objective to advocate the suitable technique and solvent for better extraction of phyto-constituents. ABSTRACT To detect and estimate the phenolics and flavonoids of Paederia foetida in different solvents under CP, MAE system and by HPTLC finger print analysis. Phenolic components in chlorophyll free extracts in different solvent groups were qualitatively detected by chemical tests and total polyphenols and flavonoids were quantitatively estimated following standard protocol. Distinct chemo-profile of the components was analyzed by HPTLC technique with CAMAG win CATS planar chromatography manager software. Among two methods of extraction more components of polyphenols were detected and significantly higher (p<0.05) contents of phenolics and flavonoids were estimated in MAE method than CP method. Out of five solvent types, organic solvent mixtures recovered significantly higher polyphenols (p<0.05) and flavonoids (p<0.001) in comparison to aqueous solvent. CP Method exhibits 12, 14, 14, 14 and 16 peaks for Gr-A, B, C, D and E solvents respectively. In MAE method the numbers of peaks in these respective solvent groups are 15, 21, 19, 18 and 22. Phyto-chemicals can be better screened qualitatively and estimated quantitatively in organic solvent extracts under MAE system by HPTLC finger print analysis than the aqueous solvent and CP method. Abbreviations: CP: Cold Percolation, MAE: Microwave-Assisted Extraction, R f : Retention factor, HPTLC: High Pressure Thin layer Liquid Chromatography. KEYWORDS Paederia foetida HPTLC finger printing polyphenol Flavonoid Received on : 21.03.2013 Accepted on : 29.05.2013 *Corresponding author