1 Mechanotransduction of ultrasound is frequency dependent 1 below the cavitation threshold 2 Tobias M. Louw, *† Gaurav Budhiraja, *† Hendrik J. Viljoen, † Anuradha Subramanian †‡ 3 The authors declare no conflict of interest. 4 5 * Both authors contributed equally to this paper and are considered co-first authors † Department of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln ‡ Corresponding author: asubramanian2@unl.edu 402-472-3463 Department of Chemical & Biomolecular Engineering, 207 Othmer Hall, 820 N. 16 th street, University of Nebraska-Lincoln, Lincoln, NE 68588-0643