American Journal of Phytomedicine and Clinical Therapeutics www.ajpct.org Original Article Screening of Fungicidal Activity of Salix and Triumfetta Species of Garhwal Himalaya S.C Sati*, Harpreet Singh, P.P Badoni and M.D Sati Department of Chemistry, HNB Garhwal University (A Central University), Srinagar, Uttarakhand ABSTRACT The aim of present study is to find out fungicidal activity of salix babylonica and triumfetta pillosa. Plant extract was mixed in sterilized distilled water to make the desired concentration (v/v) for experiments. Poisoned food technique was used to screen different plant extracts in vitro. Different concentrations (2, 5, 10, and 20%) of plant extracts were incorporated to Oat meal Agar medium for inoculation of the test pathogen in sterilized petridishes. The isolated pathogen grown on Oat meal agar medium was placed at the center of petridishes containing different concentration of the poisoned medium and incubated at 28±1°C for 8 days. Radial growth (cm) of fungus was measured after inoculation till 8 days at an interval of 24 hours Keywords: Fungicidal, salix and triumfetta species, Garhwal Himalaya, Fusarium oxysporum. INTRODUCTION The prevalence of resistance to antifungal agents significantly increased in the past decade. Resistance to antifungal agents has important implications for morbidity, mortality and health care in the community. Until recently, fungi were not recognized as important pathogens because the annual death rate due to candidiasis was steady from 1950 to 1970 1,2 Since 1970, this rate increased significantly due to more widespread use of immunosuppressive therapies, indiscriminate use of broad- spectrum antibacterial agents, the common use of indwelling intravenous devices and immuno suppressive viral infections such as AIDS. These developments and the associated increase in fungal infections 3 necessitated the search for new, safer, and more potent agents to combat serious fungal infections. For nearly 30 years, amphotericin B, which causes significant nephrotoxicity,was the sole drug available to treat serious fungal infections. The imidazoles and the triazoles in late 1980s and early 1990s were major advances in safe and effective treatment of local and systemic fungal infections. The high safety profile of triazoles, in particular fluconazole, has led to their extensive use. Fluconazole has been used to treat in excess of 16 million patients, Address for Correspondence Department of Chemistry, HNB Garhwal University (A Central University), Srinagar, Uttarakhand E-mail: sati_2009 @rediffmail.com