American Journal of Advanced Drug Delivery www.ajadd.co.uk American Journal of Advanced Drug Delivery www.ajadd.co.uk Original Article In-vivo Titanium Dioxide (TiO 2 ) Nanoparticles Effects on Chromosomal Abnormalities and Lactate Dehydrogenase Activity Javad Sharifi Rad* 1,2 , Mahsan Hoseini Alfatemi 3 , Mehdi Sharifi Rad 4,5 ,Majid Sharifi Rad 6 , Dhrubo Jyoti Sen 7 and Sasan Mohsenzadeh 8 1 Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, P.O. Box 61615-585 Zabol, Iran. 2 Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, P.O. Box 61615-585 Zabol, Iran 3 Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, P.O. Box 71455-119 Shiraz, Iran 4 Kerman university of Medical Sciences, Kerman, Iran 5 Zabol University of Medical Sciences, P.O. Box 61615-585 Zabol, Iran 6 Department of Rangeland Watershed Management, Faculty of Natural Resources, University of Zabol, Iran 7 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Gujarat Technological University, Arvind Baug, Mehsana-384001, Gujarat, India 8 Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran ABSTRACT In this study we investigated effect of TiO 2 nanoparticles on chromosomal abnormalities and lactate dehydrogenase activity and for this purpose nano anatase of titanium dioxide in four dosages of 10, 50, 100, 500, 800mg/Kg BW during intraperitoneal injection of male mice Balb/C were treated. After 24 hours, micronucleus test was carried out on the samples of bone marrow and determination of the Level of Lactate Dehydrogenase (LDH) level test performed on the samples of peripheral blood. Result showed that nano anatase of titanium dioxide in low dosage of 10, 50, 100mg/Kg BW, had not toxicity effects on bone marrow but in high dosages of 500, 800mg/Kg BW caused induced toxicity effect with increase micronucleus. In dosage of 10mg/Kg BW of nanoparticles showed LDH enzyme is blocked and a reduced in LDH level. The results of this study will be providing useful information for researcher to design of nanotechnology products Date of Receipt - 16/ 07/ 2013 Date of Revision- 19/ 07/ 2013 Date of Acceptance- 24/ 07/ 2013 Address for Correspondence Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, P.O. Box 61615-585 Zabol, Iran. E-mail: javad.sharifirad@ gmail.com