American Journal of Advanced Drug Delivery www.ajadd.co.uk American Journal of Advanced Drug Delivery www.ajadd.co.uk Original Article Studies on Inclusion Complex of 3-(2-Chloro Phenyl) 4-Methyl 2- Thiocarbamoyl -3,3a Dihydro Pyrazolo[3,4c] Pyrazole Sunakar Panda* and Rabinarayana Sahu P. G. Department of Chemistry, Berhampur University, Bhanjabihar-76007Ganjam, Odisha, India ABSTRACT The inclusion complex of 3-(2-chloro phenyl) 4-methyl 2- thiocarbamoyl-3,3a dihydropyrazolo[3,4c] pyrazole has been synthesized with β-cyclodextrin so as to improve its solubility and bio accessibility. The formation of inclusion complex has been ascertained by changes in melting point and spectral data (UV, IR, XRD, NMR etc). The determination of thermodynamic stability constant suggests that the inclusion complex has appreciable stability and its formation is thermodynamically allowed.The study of the antibacterial activity of the compound and its inclusion complex suggests that inclusion formation increases antibacterial activity significantly. Keywords: Bis-pyrazole, Inclusion-complex, β-Cyclodextrin, Aqueous phase solubility, Antibacterial activity. INTRODUCTION Pyrazole framework is an important pharmacophore and has been extensively used for the synthesis of a number of medicines such as antibacterial, antifungal, antiviral, anti-tubercular, antiamoebic etc. 1- 5 In addition, fused bispyrazoles are also reported to be a fertile resource of medicine for treating a number of diseases like cancer, malaria etc. 6 Introducing suitable functionality into these derivatives, attempts have also been made to potentiate their pharmacological activities 7 . But poor solubility of all these compounds in polar medium may be a limiting factor for reducing their bio accessibility. The solubility and hence bio accessibility can be enhanced by forming inclusion complexes with β-cyclodextrin. 8-14 In this paper, the compound 3-(2-chloro phenyl) 4-methyl 2- thiocarbamoyl-3,3a dihydropyrazolo [3,4c] pyrazole has been synthesized in its purest form by incorporating pyrazoline into a pyrazole unit and its inclusion complex has been prepared with β-cyclodextrin.Finally an attempt has been made to study the spectral, thermodynamic and antibacterial Date of Receipt - 24/ 11 / 2013 Date of Revision- 30/ 11/ 2013 Date of Acceptance- 05/ 12/ 2013 Address for Correspondence P. G. Department of Chemistry, Berhampur University, Bhanjabihar-760007 Ganjam, Odisha, India E-mail: sunakar_panda @yahoo.com