[Yadav, 2(10): October, 2013] ISSN: 2277-9655 Impact Factor: 1.852 http: // www.ijesrt.com (C) International Journal of Engineering Sciences & Research Technology [2789-2792] IJESRT INTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY A Process Development FOR Extraction of Marker Compound from EMBLICA OFFICINALIS FRUIT Saroj Yadav *1 , Anoop Kumar 2 , Om Prakash 3 *1 Institute of Pharmaceutical Sciences and Research Center, Bhagwant University, Ajmer Rajasthan, India 2 Department of Pharmaceutical Technology, MEIT Meerut, India 3 R&D Center, AVA CHCPL India rishika5936@gmail.com Abstract The present study is done to examine and optimize the process of enzyme-assisted extraction of Gallic acid from the dried fruit of Emblica officinalis. The aqueous extraction yielded 12.09% of Gallic acid. A selective extraction process after the treatment with enzymes is proposed by using 30% (v/v) methanol which releases up to 15.68% of the Gallic acid, present in the dried fruit. The optimal conditions were as follows: pH value was 4.5, concentration of cellulase solution was 2.5 mg/mL, incubation time was 8 h, incubation temperature was 50 ºC and solid:solvent ratio was 1:8 . Enzyme-assisted extraction was proven to be highly efficient in the process development for extraction of marker compounds from herbal drugs and could be used in making enriched herbal extracts of product on a large scale. Keywords: Emblica officinalis, Total Gallic acid, enzymatic extraction Introduction Emblica oficinalis is effective in the treatment of amlapitta viz.., peptic ulcer 1-2 . The fruits exhibit hypolipidaemic and antiatherosclerotic efftcts in the rabbits and rats 3-4 . The extract of amla also has antimicrobial properties 5-6 . Amla is an antioxidant with free radical scavenging properties 7 . Hepatoprotective 8 , adaptogenic 9 , antimutaginic 10 , cytoprotective and antitumor 11 antifungal 12 , were also exhibited by Amla. The Gallic acid is the basis for the quality control of Amla and other plant-derived drugs from the herb. Fig. 1. Chemical structure of Gallic acid. Although many reports about extracting Gallic acid by using different extraction methods from different plants have been published [13], some disadvantages still exist such as large organic solvent consumption, and low efficiency. Therefore, high efficient extraction method of Gallic acid represents a hot spot in Emblica officinalis research. Enzyme-assisted extraction is a method applied to the study of secondary metabolites releasing from biogenic materials. It possess the advantages of environmental- friendship, high efficiency and easy operation process. It has been represented as an alternative way for extracting marker compound from the herbal drug. Hydrolytic enzymes including cellulase, beta-glucosidase and pectinase, which are commonly used in extraction [14-16], can interact on cell wall, break down its structural integrity so as to increase the releasing of Gallic acid notably. The main aim of the present study is to examine and optimize the process of enzyme-assisted extraction of Gallic acid from the Fruits of Emblica officinalis. For this purpose, the selection of enzyme type, pH and the concertration of enzyme solution, incubation time and temprature were studied, in order to obtain high yields of above natural products economically and environmental friendly.