Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(4):171-174 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 171 Synthesis and antimicrobial activity of thiazine derivatives Sayaji S. Didwagh and Pravina B. Piste * P. G. Department of Chemistry, Yashavantrao Chavan Institute of Science, Satara, Maharastra, India _____________________________________________________________________________________________ ABSTRACT A series 2-[4-(2-amino-6-phenyl-6H-1,3-thiazin-4-yl)phenoxy]ethanol derivative (4a-4g) were synthesized from 1- [4-(2-hydroxyethoxy)phenyl]-3-phenylprop-2-en-1-one (3a-3g) with thiourea And sodium hydroxide in ethanol. All the synthesized compounds characterized on the basis of their IR, 1H NMR spectroscopic data and elemental analysis. All the compounds have been screened for antimicrobial activity by the cup-plate method. Keywords: Synthesis, Chalcones, aldehyde, thiazines, Antimicrobial activity. _____________________________________________________________________________________________ INTRODUCTION Hetrocycles are abundant in nature and involved synthesis of pharamaceuatical and biological important molecules. Thiazines and their derivatives are played important role in hetrocyclic chemistry. A large number thiazine derivatives also exhibited various biological activities such as antimicrobial [1], anti-inflammatory [2],antioxidant [3],antipyretic[4] ,antitumor [5], calcium channel modulators [6]. The Chalcones have been used as intermediates for synthesis of various heterocyclic compounds.Literature review reveals that chalcones exhibited various biological and pharamacological activities such as antimicrobial [7],antifungal [8] , analgesic [9], anti-platelet [10],insecticidal [11], anti-malarial[12], antiviral[13] activities. In view of these observations and in continuation of our work on biologically active hetrocycles and their increasing importance in pharamaceuatical and biological field[14]. The synthesis of the new antibacterial,antifungal agents to help in the battle against pathogenic microorganisms. Therefore ,we synthesized new 2-[4-(2-amino-6-phenyl-6H-1,3-thiazin-4-yl)phenoxy]ethanol derivatives and evaluated their biological activities EXPERIMENTAL SECTION The melting points were recorded on electro-thermal apparatus and are uncorrected. The purity of the compounds was checked by TLC on pre-coated SiO 2 gel (HF254, 200 mesh) aluminium plates (E Merk) using hexane and ethyl acatate visualized in iodine chamber. IR spectra were recorded in KBr on a perkin-Elmer model-983. 1HNMR spectrum recorded on Varian Mercury 300MHz instrument using CDCl 3 , DMSO-d 6 as solvent (chemical shift in δ ppm),using TMS as internal standard. Elemental analysis was performed on a Heracus CHN analyzer and was within the ±0.5% of the theoretical values. Procedure for preparation of potassium salt of p-hydroxy acetophenone (1) To an ethanolic solution of KOH (5.4 g in 72ml) ,p-hydroxy acetophenone (10 g) was added with stirring. The solution was stirred at room temperature for 1-2 hr and concentrated under reduced pressure. Diethyl ether (40 ml) was added to it. Solid of potassium salt of p-hydroxy acetophenone was separated out. It was washed with diethyl ether and kept for drying in a desiccator under vacuum. Preparation of 1[4-(2-hydroxy- ethoxy ) phenyl] ethanone (2) 2-Chloroethanol (5.52 ml) was added in potassium salt of p - hydroxy acetophenone (10 g) suspended in dry DMF (20 ml).The mixture of flaske were refluxed in the oil bath at 80 -90 0 C for 18 hr under anhydrous conditions. The