Sitapha et al Journal of Drug Delivery & Therapeutics; 2013, 3(5), 18-21 18 © 2011, JDDT. All Rights Reserved ISSN: 2250-1177 CODEN (USA): JDDTAO Available online at http://jddtonline.info RESEARCH ARTICLE COMPARATIVE STUDY CHROMATOGRAPHIC FRACTIONS ACTIVITIES FROM TERMINALIA IVORENSIS AND KETOCONAZOLE AS STANDARD ANTIFUNGAL ON IN VITRO GROWTH OF TRICHOPHYTON MENTAGROPHYTES VAR. INTERDIGITALE Sitapha Ouattara 1 *, Mathieu KRA Adou Koffi 1 , Elisée Kporou Kouassi 1 , Noël Zirihi Guédé 2 , Félix Yapi Houphouët 1 , David N’guessan Jean 1 , Joseph Djaman Allico 3 . 1 Biochemical Laboratory of Pharmacodynamics, Department of Biosciences, University Félix Houphouët Boigny Abidjan, 22 BP 582 ABIDJAN 22 (Côte d’Ivoire) 2 Laboratories of Botany, Department of Biosciences, University Félix Houphouët Boigny- Abidjan 3 Fundamental and Medical Biochemistry Unit of Pasteur Institute (Côte d’Ivoire) *Correspondence and drawn with share, E-mail: sitaphao@yahoo.fr Cell : (225) 07 46 03 46. INTRODUCTION The infectious diseases became extensive. Among them, candidosis, cryptococcosis, aspergillosis are mycosis in progression (Chabasse, 1994; Dromer and Dupont, 1996; Kra, 2001; Rosenhein and Itoua-N, 1989). This situation is related to several factors of which most projecting are: - lack of adequate medical structures; - qualified staff shortage; - lack of tools for diagnosis; - self medication; - bad using of the drugs; Even advent of HIV in human viral pathology in the years 1980 which had the most negative impact on medical evolution in under African western area. Indeed, problem of aids which carry away propagation of a great number of opportunist infections. In spite of medical drugs against mycosis, therapeutic rate of failure is high (Belhadj and al., 1994; Dupont and al., 1990). The inefficiency of current treatments led populations stripped to direct itself towards pharmacopeia plants for their cure (Adjanohoun and Aké-Assi, 1979; Ebrahim, 2003; Lorougnon, 1995; Pousset, 1989; Zirihi, 1991). In fact, medicinal plants use by populations exists since old times. More than 80% of populations use plants for their primary health care (Karou and al., 2006). However the badly using of medicinal plant could have health accidents (renal insufficiency, cardiopathies, and intoxications). To help populations from medicinal plants use, our team had work to extract active principles from medicinal plants by checking their therapeutic virtues and in order to give them scientific basis. Among many plants requested by faith healers, T. ivorensis (Combretaceae) is used against diarrheal, diabetes, hypertension, parasites and coughs. This plant is also used in treatment of cutaneous infections, buccal and teeth infections. To check these anti-infectious virtues, antifungal activities of hydroalcoholic extract of T. ivorensis is improved on the in vitro growth of Trichophyton mentagrophytes var. interdigitale. MATERIAL AND METHODS Plant Material The Plant is a powder obtained from trunk’s barks from T. ivorensis (Combretaceae) codified TEKAM 2. These barks were collected in the Nangui Abrogua University area (Abidjan-Côte d'Ivoire). Extraction The barks were collected, washed, dried with sun’s shelter at a temperature between 25 and 27°C and were returned out of powder fine with an electric crusher of IKA-MAG type. Hundred (100) grams of this powder were extracted in a mixture from solvent with 70% from ethanol and 30% from water by homogenization in Blender. After six (6) cycles of homogenization, the homogenate obtained was dried in a white fabric and was filtered successively twice on cotton and once on paper whatman 3mm. The filtrate was concentrated with a rotary evaporator of BÜCHI at ABSTRACT The present study was undertaken to evaluate in-vitro antifungal activity of Ketoconazole and extracts from trunk’s barks of Terminalia ivorensis A. Chev (Combretaceae). In vitro antifungal activity of all the extracts was done by agar slant. Extracts were incorporated in Sabouraud medium culture and extract has been inserted to this medium according to the Agar slant method and Ketoconazole were used as standards for antifungal assay. Antifungal activity was determined by diminution of mushroom in the assay tubes. For each extract five tests were done. Antifungal activity was more pronounced against Trichophyton mentagrophytes var. interdigitale. The fraction F 8 showed best antifungal activity. T. ivorensis barks extracts showed better antifungal activity than a Ketoconazole. Demonstration of antifungal activity of T. ivorensis provided the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antibiotic substances that could serve as selective agents for infectious and cutaneous diseases. This investigation has opened up the possibility of the use of this plant in drug development for human consumption possibly for the treatment of various infections caused by opportunists’ mushrooms. Keywords: Terminalia ivorensis, Antifungal Activity, chromatographic fractionation, delipidation.