S204 Document heading doi: 10.1016/S2222-1808(14)60440-0 襃 2014 by the Asian Pacific Journal of Tropical Disease. All rights reserved. Green synthesis of silver nanoparticles and its application for mosquito control Naba Kumar Mondal 1* , Arnab Chowdhury 1 , Uttiya Dey 1 , Priyanka Mukhopadhya 2 , Soumendranath Chatterjee 2 , Kousik Das 1 , Jayanta Kumar Datta 1 1 Department of Environmental Science, The University of Burdwan, Burdwan-713104, India 2 Parasitology and Microbiology Laboratory, Department of Zoology, The University of Burdwan, Burdwan-713104, India Asian Pac J Trop Dis 2014; 4(Suppl 1): S204-S210 Asian Pacific Journal of Tropical Disease journal homepage: www.elsevier.com/locate/apjtd *Corresponding author: Naba Kumar Mondal, Department of Environmental Science, The University of Burdwan, Burdwan-713104, India. Tel: +919434545694 Fax: 0342-2634200 E-mail: nkmenvbu@gmail.com 1. Introduction Mosquito have the ability of carrying and transmitting human and animal diseases across the countries causing hundreds of millions of clinical cases and millions of death annually [1,2] . Among several species of mosquitoes Culex quinquefasciatus (Diptera: Culicidae) (Cx. quinquefasciatus) is main periodic vector of filarial parasite Wuchereria bancrofti , accredited for human lymphatic filariasis transmission [3] . Cx. quinquefasciatus is a cosmopolitan mosquito with worldwide distribution, especially in the tropical and subtropical areas and is associated with human dwellings. The adult females lay eggs preferentially in relatively large, permanent aquatic habitats with high concentrations of decomposing organic matter, such as sewage effluents and septic tanks. S everal insecticides namely, DDT , dieldrin, organophosphrous, fenithothion and propoxur were widely used in I ndia to mitigate this dangerous problem [4] . Persistent application of the synthetic chemical products mostly available in local markets causes undesirable consequences including production of resistant strains of PEER REVIEW ABSTRACT KEYWORDS Silver nanoparticles, Larvicidal potential, Culex quinquefasciatus, Biological control Objective: To synthesize and characterize silver nanoparticles from aqueous root extract of Parthenium hysterophorus (P. hysterophorus) and also to evaluate the potentiality of synthesized silver nanoparticles as larvacidal agent against Culex quinquefasciatus (Cx. quinquefasciatus). Methods: The silver nano particles were generated using root extract of P. hysterophorus. The characterization of synthesized nanoparticles was done by visual color change, UV-Vis spectrum, scanning electron micrograph, fluorescent microscope and Fourier transform infrared spectroscopy. Results: It was found that aqueous silver ions can be reduced by aqueous root extract of P. hysterophorus to generate extremely stable silver nanoparticles in aqueous medium. Larvae were exposed to varying concentrations of plant extracts, aqueous silver nitrate solution and synthesized silver nanoparticles for 0, 24 and 48 h separately. Aqueous root extract showed moderate larvicidal effects; however, the maximum efficacy (60.18%) was observed with the synthesized silver nanoparticles against the larvae of Cx. quinquefasciatus. Conclusions: These results suggest that the green synthesis of silver nanoparticles have the potential to be used as an ideal eco-friently approach for the control of the Cx. quinquefasciatus. This is the first report on the mosquito larvicidal activity of the nano particle synthesized by P. hysterophorus. Contents lists available at ScienceDirect Peer reviewer Prof. Bikash Mondal, Department of C hemistry, B ejoy N arayan Mahavidyalaya, Hooghly, India. Tel: +919474778062 E-mail: bmondal.bnm@gmail.com Comments This particular research highlighted the insight of bioactive agent in the formation of nanoparticles and their possible role towards the control of mosquitoes. It has a great application in community health improvement. Details on Page S209 Article history: Received 14 Nov 2013 Received in revised form 20 Nov, 2nd revised form 24 Nov, 3rd revised form 29 Nov 2013 Accepted 12 Dec 2013 Available online 28 Jan 2014