347 Establecimiento y multiplicación in vitro de mora de castilla (Rubus glaucus meristemáticos Stablishment and in vitro multiplication of thornless blackberry (Rubus glaucus Benth.) by shoot apical meristems Alina Katil Sigarroa-Rieche † , y Claudia Lucía García-Delgado * Facultad de Ciencias Agrarias y del Ambiente, Universidad Francisco de Paula Santander, Cúcuta, Norte de Santander, Colombia. *Autor para correspondencia: clgardel23@gmail.com; †asigarroa@ufps.edu.co Rec.: 10.05.11 Acept.: 23.12.11 Resumen de micropropagación de plantas de mora (Rubus glaucus) de la variedad sin espi- nas, a partir de ápices meristemáticos. En la fase de establecimiento se evaluó un protocolo de desin-  + hipoclorito 3% con dos tiempos de exposición diferentes: T 1 por 5 min y T 2     desarrolladas se efectuó la multiplicación en los medios de cultivo M1, M3 y M4. Ambos tratamientos de desinfección resultaron efectivos alcanzando 100% de desinfección de los explantes con cada uno de -  M1 y 66.6% para M2. El análisis de varianza (Anova) y la   7.5 brotes/plántula y una altura promedio de 1.95 cm. Palabras clave: Cultivo de meristemas, cultivo in vitro, micropropagación, mora sin espinas, Rubus glaucus. Abstract propagation in a thornless variety of blackberry (Rubus glaucus) from shoot apical meristems. In the establishment phase, we evaluated a disinfection protocol: soapy solution (commercial detergent and water) for 5 minutes + 70% alcohol for 2 minutes + 3% hypochlo- rite with two different exposure times: T 1 for 5 minutes and T 2 for 10 minutes. When the microcuttings were disinfected, the meristematic shoots were removed and established in vitro in a completely ran- dom design to evaluate two cultivation mediums: M1 and M2. From the seedlings developed, the mul- tiplication was performed, which tested three cultivation mediums: M1, M3 and M4. Both disinfection treatments were effective achieving 100% disinfection of explants in each of them. Meristematic shoot  after six weeks of cultivation with rates of germination of 83.4% for M1 and 66.6% for M2. The analysis  the M1 medium favored better growth and development of the explant to obtain a multiplication coef-  Key words: In vitro cultivation, meristems, micropropagation, Rubus glaucus, thornless blackberry.