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BRITISH BIOMEDICAL BULLETIN
Original
Paraprotein-Induced Factitious Results on an Automated
Haematology Analyzer
Indira Shastry.K.*
1
, Deepak Nayak M.
1
, Chethan Manohar
1
, Ravindra Prabhu
2
and Joseph Thomas
3
1
Department of Pathology, Kasturba Medical College-Manipal Manipal University, Manipal 576104. Karnataka, India
2
Department of Nephrology, Kasturba Medical College-Manipal Manipal University, Manipal 576104. Karnataka, India
3
Department of Medical Oncology, Kasturba Medical College-Manipal Manipal University, Manipal 576104. Karnataka, India
A R T I C L E I N F O
Received 05 Feb. 2014
Received in revised form 10 Feb. 2014
Accepted 18 Feb. 2014
Keywords:
Paraproteins,
Myeloma,
Auto analyzers.
Corresponding author: Dr. Indira
Shastry.K. Department of Pathology,
Kasturba Medical College-Manipal
Manipal University, Manipal 576104.
Karnataka, India
E-mail address: Ishastry1@gmail.com
A B S T R A C T
Main findings: Paraproteins, due to their precipitating nature are
known to artifactually influence various results ranging from
haemoglobin estimation to immunological assays. We describe
herein a case of multiple myeloma on treatment and follow up,
which on routine analysis showed a spuriously high total leucocyte
and platelet count. After decanting the plasma, these parameters
receded to normal levels. The immunoglobulin assay confirmed
the paraproteins.
Summary: Analysis of blood and its components by auto
analyzers have simplified many a complex test. But few variables
have to be borne in mind while scrutinizing the suspect messages
flagged by the analyser. Some of these variables may be
inexplicably linked to the patient’s condition itself. And one of the
most common variable is paraproteins seen in patients with
multiple myeloma. This case aims to highlight some of the lesser
known causes of pre-analytical errors concerning hemoglobin
estimation by auto analyzers.
Potential implications: Paraproteins have been shown to interfere
with a number of clinical laboratory tests due to their precipitating
quality. Although seldom observed in routine diagnostic parlance,
the possible clinical implications of such phenomena must never
be overlooked.
© 2014 British Biomedical Bulletin. All rights reserved
Introduction
Paraproteins, also known as
monoclonal immunoglobulins are
proteinaceous secretions elaborated by
neoplastic clonal plasma cells. Circulating
paraproteins are intimately associated with
multiple myeloma, also with