Potravinarstvo ® Scientific Journal for Food Industry Volume 8 167 No. 1/2014 INTRODUCTION Fatty acid are important sources of body fuel because, when metabolized they yield large quantities of ATP and many cell types can use either glucose or fatty acid for this purpose in particular heart and skeletal muscle prefer fatty acid, despite longstanding assertions to the contrary, the brain can use fatty acid as a source of fuel in addition to glucose and ketone bodies (Goodhart and Shils, 1980; Marin-Valencia et al., 2012). Fatty acids have been linked with the pathological processes of the various human diseases, particularly cardiovascular disease with the strongest evidence suggesting that saturated fatty acids (SFA) have negative consequences on human health whilst polyunsaturated fatty acids (PUFA) have beneficial effects (Gibbs et al., 2013). One reason for the success of the broiler meat industry has been the consumer perception of a healthy product that contains less fat, most predominantly unsaturated fatty acids as comparable to beef or pork products (Leeson, 1999; Bonoli, 2007). That dietary monounsaturated fatty acid enrichment has a positive effect on cardiovascular health, decreasing low-density lipoprotein cholesterol, but not high-density lipoprotein cholesterol in blood plasma, and decreasing the susceptibility of low-density lipoprotein to oxidation (Grundy, 1986; Roche, 2001). The fatty acid profile of poultry meat is related to the composition of the bird’s diet and, as such, dietary alterations can be used to modify the proportion of PUFA in chicken meat (Rymer and Givens, 2005). The enrichment of poultry tissue with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) has been proposed as a potential vehicle for increasing dietary intakes of long chain n-3 PUFA in the human diet (Rymer and Givens, 2005; Gibbs et al., 2010). Bee pollen is rich of the a major fatty acid, presented as mean values were C18:3 (25.1%), C16:0 (19.6%), C18:1 (17.3%), C18:2 (8.78%), C22:0 (4.07%), and C18:0 (2.96%) acids. The proportions of C18:3 were generally higher than those of C18:2 and the ratio of total unsaturated fatty acid (TUS) to total saturated fatty acid (TS) was >1.0, except for Nelumbo nucifera Gaertn, pollen for the characteristic absence of C18:3 acids (Yang et al., 2013). The present experiment was objected to study the effect of the bee pollen extract on the broiler Ross 308 breast and thigh muscles essential fatty acids. MATERIAL AND METHODOLOGY The experiment has been done in the test poultry station of Slovak University of Agriculture in Nitra. The tested chickens were broiler Ross 308. The experiment included 90 chicks in one day-old, which were divided into 3 groups (n=30): control group, E1 and E2, for 42 days. The chickens were bred in a cage conditions. Each cage was equipped with feed dispenser and water intake was ensured ad libitum through a self-feed-pump. The temperature was controlled during the fattening period and Potravinarstvo, vol. 8, 2014, no. 1, p. 167-171 doi:10.5219/374 Received: 4 April 2014. Accepted: 9 April 2014. Available online: 25 June 2014 at www.potravinarstvo.com © 2014 Potravinarstvo. All rights reserved. ISSN 1337-0960 (online) EFFECT OF BEE POLLEN EXTRACT AS A SUPPLEMENTAL COMPONENT OF DIET ON BROILER´S ROSS 308 BREAST AND THIGH MEAT MUSCLES FATTY ACIDS Peter Haščík, Ibrahim Elimam, Jozef Garlík, Marek Bobko, Jana Tkáčová, Miroslava Kačániová ABSTRACT The present study was aimed to study the effect of the bee pollen extract on the broiler Ross 308 breast and thigh meat fatty acids. The experiment enrolled 90 chicks in one day old, which were divided into 3 groups (control, E1 and E2). The broiler has been bred in a cage condition for 42 days. To the experimental groups were added bee pollen extract in the amount (400, 800 mg.kg -1 ). The chickens have been bred in a cage conditions, each cage was equipped with feed dispenser and water intake was ensured ad libitum through a self feed-pump. The temperature was controlled during the fattening period and it was 33 °C at the first day and every week was reduced about 2 °C the end temperature was 23 °C. At the end of the experiment the fatty acids have beenevaluatedby using Agilent 7890A Gas Chromatograph apparatus (USA). The findings have been shown that the myristoleic acid, linoleic acid, linoelaidic acid, arachidonic acid, and archaic acid were decreased after using the bee pollen into broiler feed mixture otherwise, the bee pollen has been increased the polemic acids and oleic acid and there were found no significant differences (P ≥0.05) among all the experimental groups.From the recent experiment, we conclude that bee pollen extract has decreasedthe fattyacids except palmitoleic acid acid and oleic acid, whichwere higher compared to control groupand there were no significant differences (P ≥0.05) between experimental groups. Keywords: broiler Ross 308; bee pollen; feed mixture; fatty acid