g editor@tjprc.or www.tjprc.org THE EFFECT OF MIXTURE (SIDR EXTRACT WITH HYDROGEN PEROXIDE) IN THE DISINFECTION OF HOSPITAL INSTRUMENT ANWAR KADHIM AL-SAFFAR Assistant Professor, Microbiology, College of Sciences, Babylon University, Hillah, Iraq ABSTRACT Disinfection means the use of a chemical procedure that eliminates virtually all recognized pathogenic microorganisms but not necessarily all microbial forms (e.g., bacterial endospores) on inanimate objects. Factors that affect the efficacy of both Disinfection include prior cleaning of the object; organic and inorganic load present; type and level of microbial contamination; concentration of presence of biofilms; Temperature and pH of the Disinfection process; and in some cases, relative humidity of the sterilization process (e.g., ethylene oxide). To assess mixing Solution in combating bacterial infection of reusable medical advices and surgical instrument. In this study we used Eighteen pieces of medical advices including 5 tubing system of respirator,5 face masks, 6 laryngoscope blades, 2 stethoscopes (bell and diaphragm parts) of previously using that was collected from Teaching Surgical Hospital in the Hilla City. Rinsed these pieces twice with normal saline. The present study designed to four groups: group one including different weight of sidr leaves aqueous extract 25, 50, 62.5 and 75 g/L, group two: we used 1.5% Hydrogen peroxide only, group three: a mixture of each extracts and 1.5% Hydrogen peroxide were well mixed together, and group four normal saline 0.9% was used as a control. The experiments of decontamination were performed at different times 5, 10, 15 and 30 min for each groups. Swabs were taken from each advice before and after contact with the disinfectants. The result of present study, showed the mixing 1.5% Hydrogen peroxide and 62.5 g/L sidr was effective in disinfection of used contaminated medical advices at 30 min. KEYWORDS: Sidr, Disinfection, Hydrogen Peroxide, Medical a Devices INTRODUCTION Reusable devices or items that touch mucous membranes should, at a minimum, receive high-level disinfection between patients. Because the vast majority of pathogens are present in organic matter, e.g. visible soil, the first step in reprocessing, cleaning, is the most important 1,2 Any failure to remove soil at this point creates the potential for transmission of infection as the efficacy of subsequent disinfection or sterilization will be compromised. Decontamination is the process by which microorganisms are removed or destroyed in order to render an object safe 3 . Cleaning is the first and most important step in the decontamination process and involves the removal, usually with a detergent and water, of both visible and non-visible soil (such as blood, protein substances, and other debris) from the surface of the medical device. Failure to properly disinfect or sterilize reusable medical equipment carries a risk associated with breach of the host barriers 4 . Nosocomial infections- known also as hospital-acquired infections, hospital-associated infections, and hospital infections-are infections that are not present in the patient at the time of admission to hospital but develop during the course of the stay in hospital 5 . International Journal of Botany and Research (IJBR) ISSN(P): 2277-4815; ISSN(E): 2319-4456 Vol. 4, Issue 3, Jun 2014, 7-10 © TJPRC Pvt. Ltd.