International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol. 3, No.3, pp 1403-1407, July-Sept 2011 Analytical Method Development and Validation of Lafutidine in Tablet dosage form by RP-HPLC M.Sumithra*, P.Shanmuga Sundaram, K.Srinivasulu, Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Vels University, Chennai-600117,India. *Corres.author: sumi_apcp@yahoo,com Abstract: A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of lafutidinine in tablet dosage form. Hypersil silica, C 18 250× 4.6 mm. 5µ. column. was used with a mobile phase containing a mixture 0.02m dihydrogen potassium ortho phosphate,0.02m dipotasium hydrogen ortho mphasphate(p H 6) and Acetonitrile and in the ratio of 30:70. The flow rate was 1.0ml/min and effluents were monitored at 215nm and eluted at 7.75min respectively. Calibration curve was plotted with a range from 27-81μg/ml. The assay was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The proposed method can be useful in the routine analysis for the determination on lafutidinine in tablet dosage. Key words: lafutidinine in tablet dosage, Reverse phase HPLC, 0.02m dihydrogen potassium ortho phosphate,0.02m dipotasium hydrogen ortho mphasphate. INTRODUCTION lafutidine 2-[(2-furylmethyl)sulfinyl]-N-((2Z)-4-{[4- (piperidin-1- ylmethyl)pyridin-2-yl]oxy}but-2-en-1- yl) acetamide are used in the treatment of anti ulcer. Structures are shown in fig 1 respectively. Antisecretory drugs are used in the treatment and prophylaxis of peptic ulcer disease some are also employed in other disorders associated with gastric hyperacidity such as gastro-oesophageal reflux disease and dyspepsia . They may be divided into Histamine H2-receptor antagonists (H 2 -antagonists), which act by blocking histamine H 2 -receptors on gastric parietal cells, thereby antagonising the normal stimulatory effect of endogenous histamine on gastric acid production Fig 1: Structure of lafutidine