Int.J.Curr.Microbiol.App.Sci (2014) 3(4): 161-171 161 Original Research Article Evaluation of phytochemical and in vitro anti-oxidant, anti proliferative activity of a polyherbal Siddha formulation Vallarai nei M.Ravichandran 1* and H. Mubarak 2 1 Department of Gunapadam (UG), GSMC, Palayamkottai, India 2 Senior Research Fellow(S), SCRU, Palayamkottai, India *Corresponding author ABSTRACT Introduction Siddha system is one of the pioneer systems of medicine among traditional medicine practices in India (Sambasivam Pillai,1993). There are many formulations prescribed by the ancient Siddhars. Unique way of prescribing medicines by this system draws attention worldwide for keen research in drugs for reverse pharmacology manner. In this modern era there are number of new drug inventions are going day by day and substituting the previous generation drugs in order to empower the health systems. But the age old systems are blended with natural principles remains unchanged. The philosophy behind this system is bound with humane and nature of the universe. In Siddha system of medicine, the line of treatment goes with single drug therapy to compound drug formulations which are prescribed in classical literature as curative ailments for many diseases. WHO ISSN: 2319-7706 Volume 3 Number 4 (2014) pp.161-171 http://www.ijcmas.com Keywords Siddha; Vallarai nei; MTT assay In India, Cervical cancer occurrence in women is of high incidence rate that of 1, 30,000 patients are suffering among them 70,000-75,000 deaths occur annually. Vallarai nei (VN) a commonly known Siddha drug is prescribed by Siddha physicians for various ailments in clinical practice. In siddha literature Vallarai nei (VN) is indicated for Yoni putru ( Cervical cancer).The objective of this study is to analyze scientific basis for the anti oxidant, anti proliferative property of VN on HeLa cell lines were evaluated in vitro by employing MTT assay. Preliminary phytochemical analysis was done to identify the presence of constituents. The crude extract exhibited cytotoxic effects on HeLa cell lines which correlates with the indication mentioned in the Siddha pharmacopeia. The IC 50 value obtained from the MTT assay was 51.6 ug/ml. The antioxidants activity assessed by different methods exhibited the presence of significant activity. The phytochemical screening revealed the presence of phenols and terpinoids which are useful in controlling the cancer cellular pathways. At the end the combined activities analyzed giving hope for prescribing the VN to the needy patients.