Hindawi Publishing Corporation BioMed Research International Volume 2013, Article ID 703626, 7 pages http://dx.doi.org/10.1155/2013/703626 Research Article Schiff Base Metal Derivatives Enhance the Expression of HSP70 and Suppress BAX Proteins in Prevention of Acute Gastric Lesion Shahram Golbabapour, 1,2 Nura Suleiman Gwaram, 3 Mazen M. Jamil Al-Obaidi, 4 A. F. Soleimani, 5 Hapipah Mohd Ali, 3 and Nazia Abdul Majid 2 1 Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia 2 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 3 Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 4 Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, Malaysia 5 Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Kuala Lumpur, Malaysia Correspondence should be addressed to Nazia Abdul Majid; nazia@um.edu.my Received 26 June 2013; Accepted 22 September 2013 Academic Editor: Ibrahim Banat Copyright © 2013 Shahram Golbabapour et al. his is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Schif base complexes have appeared to be promising in the treatment of diferent diseases and disorders and have drawn a lot of attention to their biological activities. his study was conducted to evaluate the regulatory efect of Schif base metal derivatives on the expression of heat shock proteins (HSP) 70 and BAX in protection against acute haemorrhagic gastric ulcer in rats. Rats were assigned to 6 groups of 6 rats: the normal control (Tween 20 5% v/v, 5 mL/kg), the positive control (Tween 20 5% v/v, 5 mL/kg), and four Schif base derivative groups named Schif 1, Schif 2, Schif 3, and Schif 4 (25 mg/kg). Ater 1 h, all of the groups received ethanol 95% (5 mL/kg) but the normal control received Tween 20 (Tween 20 5% v/v, 5 mL/kg). he animals were euthanized ater 60 min and the stomachs were dissected for histology (H&E), immunohistochemistry, and western blot analysis against HSP70 and BAX proteins. he results showed that the Schif base metal derivatives enhanced the expression of HSP70 and suppressed the expression of BAX proteins during their gastroprotection against ethanol-induced gastric lesion in rats. 1. Introduction Wide spectrum applications of the Schif base in biological systems have opened a new horizon in pharmaceutical researches. Schif bases are usually synthesized through the condensation process of primary amines and active carbonyl groups. Furthermore, indole derivatives have critical roles in variety of biological activities. In novel therapeutics, the development of hybrid molecules consisting diferent pharmacophores in one frame may lead to remarkable phar- macological efects. he coadministration of the moieties, acting by diferent mechanisms, may have a synergistic efect with higher activities [1]. he pharmacological activity of Schif bases metal complexes is solely attributed to the metal, its ligands, or both. Two important factors such as maximum thermodynamic stability and large degree of selectivity are crucial in the design of Schif base metal complexes or ligands for pharmaceutical application. Various metal com- plexes have been introduced for their potential therapeutic applications. Possessing various biological activities, some of the irst row transition metals are important in metallo- proteins. he chemistry of the metal complexes of Schif bases containing nitrogen and other donors is well described by Tarafder and colleagues [2]. Various Schif base complexes have shown variety of properties such as anticancer [3–5], antimicrobial, antifungal [6, 7], antiviral [8] and antioxidant, and anti-inlammatory activity [9]; also see [10]. It is believed that some complexes are more efective in metal complexes