GEETHA NATESAN et al, International Journal of Pharmaceutical Sciences and Business Management,
Vol.2 Issue. 9, September- 2014, pg. 26-35 ISSN: 2310-6913
© 2014, IJPSBM All Rights Reserved, www.ijpsbm.com 26
PHYTOCHEMICAL EVALUATION OF
PEEL OF CITTRUS RETICULATA BLANCO
USING VARIOUS SOLVENT EXTRACTS
JERLIN SHOWMYA JUSTIN
1
, ARCHANA MILTON
2
, GEETHA NATESAN*
1, 2
Research Scholars and * Professor and Head
Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal,
Tamil Nadu, India
Email: showmyajerlin@yahoo.com, archuadmire@gmail.com, geethadrbio@gmail.com
ABSTRACT
Objectives: The aim of the present study is phytochemical evaluation of peel of Citrus
reticulata using various solvent extract.
Methods: In the present investigation both qualitative and quantitative phytochemical
analysis were carried out as per standard procedures.
Results: Phytochemical screening reveals the presence of tannins, phenolics,
flavonoids, steroids, glycosides and terpenoids in ethanolic and methanolic extracts, where as
glycosides is absent in acetone extract and all the present compounds are found in very
minute level. Of the three extract tried for the phytochemical screening methanolic extract
showed the maximum number of bioactive compounds followed by ethanol and acetone.
Conclusion: Result approves the presence of phytochemical compounds and amount
of primary and secondary metabolites in three extracts of Citrus reticulata peel. Among the
three extracts methanolic extract showed high level of bioactive compounds compared to
other two extracts. This is the first report using peel extracts of Citrus reticulata. From the
results it is clear that determination of biological active compounds from plant materials is
largely dependent on the type of solvent used in the extraction procedure.
Key words: Phytochemical, Bioactive compounds and Citrus reticulata