1 PENGARUH IMPLEMENTASI BUDAYA TRI HITA KARANA TERHADAP PENGGUNAAN SISTEM INFORMASI AKUNTANSI DIMEDIASI KEYAKINAN-DIRI ATAS KOMPUTER, KEINOVATIFAN PERSONAL, PERSEPSI KEGUNAAN, DAN PERSEPSI KEMUDAHAN PENGGUNAAN PADA BANK PERKREDITAN RAKYAT DI BALI I MADE SADHA SUARDIKHA ( Universitas Udayana ) SUTRISNO T. EKO GANIS SUKOHARSONO BAMBANG PURNOMOSIDHI ( Universitas Brawijaya ) Abstract This study aims to examine and analyze the influence of Tri Hita Karana (THK) culture on the use of Accounting Information System (AIS), either directly or indirectly mediated by the main Technology Acceptance Model (TAM) variables (perceived usefulness and perceived ease of use) and external variables of TAM (computer self-efficacy and personal innovativeness); the influence of THK culture on computer self-efficacy, and personal innovativeness; influence perceived usefulness and perceived ease of use on the use of AIS as an expression of the success of AIS. This research is located in Bali with the object of Rural Banks (Bank Perkreditan Rakyat). Variables examined consisted of six, namely: THK Culture, computer self-efficacy, Personal innovat- iveness, Perceived usefulness, Perceived ease of use, and Use of AIS. This study look at the entire population of Rural Banks in Bali. Required data collected by census using a list of questions asked to the respondents. The data has been collected was analyzed using an analysis tool SEM, PLS Smart 2.0 M3 approach. The results of this study show that: 1) THK culture influences computers self-efficacy, personal innovativeness, and the use of AIS as an expression of the success of AIS; 2) THK Cultural affect the use of AIS is mediated by computer self-efficacy, personal innovativeness, perceived usefulness, and perceived ease of use; 3) Computer self-efficacy affects the perceived usefulness and perceived ease of use; 4) Personal innovativeness affects perceived usefulness and perceived ease of use; and 5) the perceived usefulness and perceived ease of use affect the use of AIS. Keyword: THK Culture, Computer self-efficacy, Personal innovativeness, Perceived usefulness, Perceived ease of use, and Use of AIS.