Global Veterinaria 13 (6): 1043-1050, 2014 ISSN 1992-6197 © IDOSI Publications, 2014 DOI: 10.5829/idosi.gv.2014.13.06.9187 Corresponding author: Ahmed Hegazi, Department of Zoonotic Diseases, National Research Center, Egypt. E-mail: ahmed@ahmedhegazi.com - ahmedhegazi128@gmail.com. 1043 In vitro Effects of Some Bee Products on T. gondii Tachyzoites Ahmed G. Hegazi, Hassan A. El-Fadaly, Ashraf M. Barakat and Sanaa K.A. Abou-El-Doubal 1 1 1 2 Department of Zoonotic Diseases, National Research Center, Dokki, Giza, Egypt 1 Department of Parasitology and Animal Diseases, National Research Center, Egypt 2 Abstract: Toxoplasma gondii, is ideal opportunistic zoonotic protozoan, exceeds neurological and congenital impact sequence to flare up of latent toxoplasmosis. It is intracellular pathogen and is so difficult to treat, it requires higher doses of intracellular antimicrobial drugs which reflect repulsive side actions. So, the aim of a current study is focused to evaluate the effect of some bee products (propolis, honey and bee venom) with special emphasis to sulfa-dugs as anti-Toxoplasma activity. The results revealed that all investigated bee products showed destructive on viable tachyzoites which promoted directly with the different dilutions of the bee products, in particular; 100, 50, 25 and 10 respectively, at chilling temperature (4°C) and corresponding to LD & LD . Bee venom proofed the faster and powerful in vitro anti-tachyzoites action with all dilutions, 50 100 exceeding even the control sulfonamides. 5, 6, 7 and 8 respectively while diluted propolis were effective to enhance the persisting viable tachyzoites course. Where 7% dilution of propolis symbolizes the maximum optimal value, successfully increases the average percent of LD &LD viable tachyzoites longer time a live 50 100 up to 172.9 hours post exposure to propolis, exceeding about three days over the normal control saline solution. In conclusion: the results revealed that bee products are promising for development of potent and novel anti- toxoplasma drug. Key words: In vitro T. gondii Tachyzoites Propolis Bee Honey and Bee Venom INTRODUCTION MATERIALS AND METHODS Toxoplasmosis is typical zoonoses, may be acute via Toxoplasma gondii Strain: In the present study the RH tachyzoites or chronic through bradyzoites stages [1]. Acute tachyzoites stage is exceeding opportunistic human impact sequence to cerebral [2], congenital [3] and ocular toxoplasmosis [4 5] and ranks among the 10 most opportunistic and the forth deadly pathogen in immune- suppressed patients [6]. Apitherapy or the medical uses of bee products are ranged from use as honey [7- 10], pollen [11], royal jelly [12], wax [13], propolis [10, 14] and venom [15, 16]. Treatment of acute human toxoplasmosis is so difficult, where higher doses of intracellular antibiotics or sulfonamides reveal terrible side actions [17]. Thus the aim of the present study is to evaluate bee products; honey, propolis and bee venom against T. gondii tachyzoites stage as safe natural materials with reference to the chemical drug sulfonamides. strain was maintained and secured in Zoonotic Diseases Department, National Research Center, Egypt, via using regular mice peritoneal passage every 3 days for continuous collection of fresh tachyzoites [18]. Animals: Forty mice were obtained from Laboratory Animals House, National Research Center, Egypt. These animals were used as long as the term of the study, housed in standard environmental conditions at temperature (24°C) and relative humidity (50%) with a 12:12 light: dark cycle. with free access to a standard commercial diet and water. All animals were used for harvesting and secure the peritoneal RH tachyzoites with continuous regular flow, by successive intraperitoneally tachyzoites-tachyzoites mice cycle every 72 hours. Experiments were performed according to the Guide for