Synthesis and characterization of amphiphilic diblock copolymers of 2-(1-imidazolyl)ethyl methacrylate and styrene Maria Rikkou-Kalourkoti, Panayiota A. Panteli and Costas S. Patrickios * Six amphiphilic diblock copolymers based on the hydrophilic monomer 2-(1-imidazolyl)ethyl methacrylate (ImEMA) and the hydrophobic monomer styrene (Sty) were prepared by sequential reversible addition fragmentation chain transfer (RAFT) polymerization. RAFT polymerization was performed via the use of 4-cyano-4-(dodecylsulfanylthiocarbonylsulfanyl)pentanoic acid (CDP) as the chain transfer agent and glacial acetic acid as the solvent. Amphiphilic diblock copolymers with a range of compositions were prepared, with ImEMA rst blocks of constant (nominal) degree of polymerization (DP) equal to 100, and Sty second blocks of a DP between 18 and 482, having ImEMA contents between 15 and 80 mol%, as measured by 1 H NMR spectroscopy in CDCl 3 . Only the Sty-rich ($51 mol%) diblock copolymers were fully soluble in the solvent for gel permeation chromatography (GPC), tetrahydrofuran; GPC analysis of these copolymers indicated molecular weights in the range of 15 to 34 kDa, and molecular weight dispersities ranging from 1.1 to 1.6. At room temperature, the side-group of the ImEMA units was stable in an acidic environment but hydrolyzed slowly under alkaline conditions. In bulk neutral samples, the ImEMA side-group was thermally stable up to 250 C. Finally, dynamic light scattering and polarized light microscopy indicated that the Sty-rich diblock copolymers formed spherical micelles in chloroform, whereas all diblock copolymers formed anisotropic nanophases in water. Introduction Imidazole is a heterocyclic aromatic molecule bearing two nitrogen atoms, conferring to it high polarity. 13 The imidazole ring has great biological importance, appearing in many bio- logical molecules including the side-group of histidine which is the main component of histones, and is part of the serine catalytic triad found in a certain type of proteases. 4 Because of its biological importance, the imidazole ring has been intro- duced in many types of synthetic polymers, including vinylics, 13,510 methacrylates 1115 and styrenics. 16 Apart from the polymerization of monomers bearing neutral imidazole side- groups, the polymerization of monomers with pendant imida- zolium salts has also been reported and currently receives great attention because of their action as ionic liquids. 1722 In most cases, imidazole has been introduced via its vinylic monomers, such as N-vinylimidazole (NVIm) or 4-vinyl- imidazole (4VIm). For example, NVIm was used for the prepa- ration of its linear homopolymers, 3 its amphiphilic random copolymers with ethyl methacrylate (EMA), 5 and its randomly- crosslinked amphiphilic copolymer conetworks with poly- (tetrahydrofuran) 6,7 using free radical polymerization, as well as the preparation of double-hydrophilic diblock copolymers with N-isopropylacrylamide 8 and amphiphilic diblock copolymers with styrene 10 using reversible additionfragmentation chain transfer (RAFT) polymerization. Regarding the other vinylic imidazole-containing monomers, Long et al. reported the use of 4VIm 2,9 for the synthesis of linear homopolymers and diblock and ABA triblock copolymers with di(ethylene glycol)methyl ether methacrylate, 9 using RAFT polymerization. There is also one example of a styrenic imidazole-containing monomer used for the preparation of random copolymers with n-butyl acrylate using nitroxide-mediated radical polymerization (NMP). 16 Additionally, imidazole-containing methacrylate monomers have also been prepared and polymerized. In particular, 2-[(1- imidazolyl)formyloxy]ethyl methacrylate was used for the preparation of its homopolymers, 11 and 2-(1-imidazolyl)ethyl methacrylate (ImEMA) was used for the preparation of its homopolymers, 12 its double-hydrophilic diblock copolymers with 2-(dimethylamino)ethyl methacrylate, 13 its ampholytic diblock copolymers with methacrylic acid, 15 and its end-linked homopolymer networks, 14 by free radical polymerization 11 and group transfer polymerization (GTP). 1215 Finally, imidazole- bearing (co)polymers were recently prepared by Lowe and coworkers 23 via a polymer modication strategy, and, in partic- ular, through the acyl substitution reaction of penta- uorophenyl acrylate units with histamine [2-(1H-imidazol-4-yl)- ethanamine]. Herein, we report for the rst time the use of ImEMA for the synthesis of its amphiphilic diblock copolymers Department of Chemistry, University of Cyprus, P. O. Box 20537, 1678 Nicosia, Cyprus. E-mail: kalourk@ucy.ac.cy; ppante02@ucy.ac.cy; costasp@ucy.ac.cy Cite this: DOI: 10.1039/c4py00221k Received 15th February 2014 Accepted 24th March 2014 DOI: 10.1039/c4py00221k www.rsc.org/polymers This journal is © The Royal Society of Chemistry 2014 Polym. Chem. Polymer Chemistry PAPER