Siddha Reviews Siddha Reviews 2015 (1) (1) Research Article IN –VITRO STUDIES OF THE CYTO TOXICITY EFFECT OF A POLY HERBAL SIDDHA FORMULATION IN BREAST CANCER CELLS C.Suvetha 1 , Thomas M.Walter 2 , R. Sweety Nirmala 3 1 Internee, Government Siddha Medical College, Palayamkottai, India. Sweths66@gmail.com 2 Assistant Professor, Department of Gunapadam,Government Siddha Medical College, Palayamkottai, India. dr.thomaswalter@walter@gmail.com 3 CEO, Bethesda Siddha Research Center, Tirunelveli, Tamilnadu. bethesdacam@gmail.com ABSTRACT Breast Cancer is a frightening disease. It remains a significant, scientific, clinical and societal challenge. Breast cancer will strike one in every eight American women. Globally, breast cancer in women with an estimated 1.38 million new cases per year 50000 cases in women was recorded each year in UK alone. There are 4,58,000 deaths per year from breast cancer worldwide making it the most common cause of female cancer death in both the developed and developing world. In the UK, the age standardized incidents of breast cancer in women has increased by 6% over the last decade, between 1999 to 2001 and 2008 to 2010.It is estimated that around 550,000-570,000 people are living with or after diagnosis of breast cancer in the UK and based on current projections, this figure is expected to triple by 2040 due to ageing population and continued improvements in survival. Based on the clinical and theoretical knowledge attained through various Siddha literatures, the Authors’ of this paper has created a formulation for the treatment of Breast cancer. This formulation is being scientifically evaluated through in-vitro anti-proliferative studies in Breast cancer cells. The study result reveals the highest efficiency of the trail drug in killing breast cancer cells. KEYWORDS Chemotherapeutic drug, Adriamycin, Cell proliferation, Breast cancer cells