International Journal of Scientific and Research Publications, Volume 4, Issue 1, January 2014 1 ISSN 2250-3153 www.ijsrp.org Chemical Analysis of Leaf Extracts of Calotropis procera. Dr. Amit Tiwari * , Sanchita Singh * , Suchita Singh ** * Department of Biotechnology Govt. T.R.S. College. Rewa India ** A.P.S. University Rewa India Abstract- Since ancient time plants have been used as source of therapeutic agents, plants play a significant role in the indigenous system of medicines to combat diseases. Plants are richest source of bioactive organic chemicals on earth. Phytochemical properties of leaf of Calotropis procera obtained from methanol and petroleum ether extracts were investigated .The results suggest that the Phytochemical properties of the leaf for using various ailments. Index Terms- Calotropis procera, Phytochemical I. INTRODUCTION hyto” is the greekword for plants. There are many “families” of phytochemicals which helps the human body in variety of way. Phytochemical are non-nutritive plant chemicals that have protective or disease preventive properties. Plant produce these chemicals to protect themself but recent research demonstrate that many phytochemicals can protect humans against disease (Srivastava et al, 2010). Calotropis procera commonly known as Aak is used in many Ayurvedic formulations like Arkelavana. The medicinal potential of Calotropis procera has been known to traditional system of medicine . The use of the plants, plant extracts and pure compounds isolated from natural sources has always provided a foundation for modern pharmaceutical compounds ( Murti et al, 2012). Plants have been a rich source of medicines because they produce wide array of bioactive molecules, most of which probably evolved as a chemical defence against predation or infection (Ramaprabha et al, 2012). It is estimated that only one percent of 2,65,000 flowering plants on earth have been studied exhaustively for their chemical composition and potential against important medicinal value (Cox et al, 1994). Here an attempt has been made to investigate the chemical present in the leaf for curing various diseases. II. MATERIAL AND METHODS: 1. Plant material: Calotropis procera leaf collected in January 2013 from Rewa. The plant material was identified at the field using standard keys and descriptions. 2. Method of extraction: Solvent – Petroleum ether, Methanol Method – Maceration Procedure: Leaf powder was weighed 500 gm and kept in a container in contact with pet ether for seven days, with vigorous shaking at regular interval. Material was filtered a first with muslin cloth and then with filter paper. Filtrate was collected and dried in water bath till no further reduction in mass of extract was observed. Dried extract was weighed and packed in air tight container and the marc was air dried then kept in a container in contact with methanol for seven days, with vigorous shaking at regular interval. Material was filtered a first with muslin cloth and then with filter paper. Filtrate was collected and dried in water bath till no further reduction in mass of extract was observed. Dried extract was weighed and packed in air tight container. 3. Phytochemical Screening- Phytochemical Screening was carried out using standard methods to detect the bioactive compounds like alkaloids, tannins, phenols, steroids, flavonoids, saponins (Trease et al, 1989). III. RESULT AND DISCUSSION “P