16 CTJPILS, Vol. 1, Issue 1, 2015 CT International Journal of Pharmaceutical and Integrated Life Sciences ISSN (online): 2395 - 0773 www.ctgroup.co.in Isolation and Arsenic Uptake Study by Immobilized LAB Cells Mandeep Sidhu* 1 , Promila Sama 2 , Hina Rehan 2 , Sheelendra M Bhatt 1 1 Department of Biotechnology and Biosciences, Lovely professional University, Phagwara-144411, Punjab, India 2 Department of Biotechnology and Biosciences, BIS Institute of Science and Technology, Moga- 142043, Punjab, India mahi.mandeep@yahoo.com ARTICLE INFO: ABSTRACT Article history: Received: 8 April, 2014 Revised: 13 August, 2014 Available online: 5 Feb, 2015 The aim of the current study was to develop effective bioremediation of Arsenic from drinking water by using immobilized arsenic resistance Lactic Acid Bacteria (LAB). For this Arsenic resistant LAB have been isolated from curd samples using spread plate technique in MRS agar media at high arsenic concentration ranging from 50 μg/ml - 1000 μg/ml. In addition, uptake of arsenic by immobilized LAB cells have also been studied by Langmuir adsorption isotherm and we had observed that immobilized LAB cells were able to absorb up to 88.14% of arsenic in 5 hours and 30 minutes. © 2015 CTES. All rights reserved Keywords: LAB, As, MRS, Bioremediation, Heavy metals I. INTRODUCTION Arsenic toxicity now posing dangerous health impacts word wide [5] not only to human health but also to broader ecosystem noted during the last few decades via ground water pollution and is said to be one of the notoriously poisonous to multicellular life [15]. Presence of excess Arsenic in the ecosystem is due to various anthropogenic sources such as production of pesticides, herbicides, and insecticides which remain persistent due to its compound formation with phosphate and nitrogen. Heavy metal pollution in not only affects the production and prominence of crops, rather extended pressure over the quality of the atmosphere and water bodies have been reported in various literature. The accumulation of heavy metals in water and soil is alarming due to the safety issues of drinking water and food. The most commonly reported heavy metal contaminants are arsenic, phosphate and fluoride [13]. Increasing Arsenic content in water has become as one of the major challenge in the world. Not only humans but animals are also coming in contact by eating grasses and crops contaminated with arsenic pesticides [14]. Toxicity of arsenic is dependent on its oxidation states. Arsenites (As III) is ten times more toxic then arsenate (As V) [19, 6]. Arsenic is reported to be mutagenic and carcinogenic [9] and is now has been reported from ground water table of west Bengal, Bangladesh, West Bengal, Punjab, Jharkhand, Bihar, Uttar Pradesh the Ganga River; Assam and Manipur in