ORIGINAL PAPER Cupriavidus yeoncheonense sp. nov., isolated from soil of ginseng Priyanka Singh • Yeon-Ju Kim • Ngoc-Lan Nguyen • Van-An Hoang • Johan Sukweenadhi • Mohamed El-Agamy Farh • Deok-Chun Yang Received: 30 September 2014 / Accepted: 23 December 2014 / Published online: 19 February 2015 Ó Springer International Publishing Switzerland 2015 Abstract A novel bacterial strain, DCY86 T (=KCTC 42053 T = JCM 19890 T ) was isolated from soil of a ginseng field in Yeoncheon province (38°04 0 00 00 N 126°57 0 00 00 E), Republic of Korea using a serial dilution method. Strain DCY86 T was observed to be Gram-stain negative, strictly aerobic, to grow optimally at 25–30 °C, at pH 7–7.5 and on tryptic soya agar medium. The cells were found to be sensitive to ceftazidine and tetracycline. Based on 16S rRNA gene sequence comparisons, strain DCY86 T was found to be most closely related to Cupriavidus basilensis LMG 18990 T (98.48 %), Cupriavidus numazensis LMG 26411 T (98.34 %), Cupriavidus pinatabonesis KCTC 22125 T (98.34 %) and Cupriavidus laharis KCTC 22126 T (98.00 %). The G?C content was determined to be 64.23 mol %. The only isoprenoid quinone detected in strain DCY86 T was ubiquinone Q-8. The major polar lipids were identified as diphosphatidylglycerol, phos- phtidylethanolamine, phosphatidylglycerol, unidenti- fied aminophosphoglycolipids and unidentified phospholipids. The major fatty acids were identified as C 16:0 summed feature 3 (C 16:1 x7c/x6c and/or iso-C 15 : 0 2-OH) and summed feature 8 (C 18:1 x7c and/or C 18:1 x6c). These data support the affiliation of strain DCY86 T to the genus Cupriavidus. Strain DCY86 T was also found to be able to solubilize phosphate and produce siderophores. The results of physiological and biochemical tests enabled strain DCY86 T to be differ- entiated genotypically and phenotypically from the recognized species of the genus Cupriaividus. There- fore, the novel isolate can be considered to represent a novel species, for which the name Cupriavidus yeon- cheonense sp. nov. is proposed here. The type strain is DCY86 T (=KCTC 42053 T = JCM 19890 T ). Keywords Cupriavidus yeoncheonense Á Proteobacteria Á Ginseng Á Polyphasic taxonomy Introduction The genus Ralstonia was proposed in 1995 to accom- modate the misplaced species Burkholderia pickettii, Electronic supplementary material The online version of this article (doi:10.1007/s10482-014-0369-z) contains supple- mentary material, which is available to authorized users. P. Singh Á Y.-J. Kim Á N.-L. Nguyen Á V.-A. Hoang Á D.-C. Yang Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Gyeonggi-do, Yongin-si 446-701, Republic of Korea Y.-J. Kim (&) Á J. Sukweenadhi Á M. E.-A. Farh Á D.-C. Yang (&) Graduate School of Biotechnology, Ginseng Genetic Resource Bank, College of Life Science, Kyung Hee University, Yongin 446-701, Republic of Korea e-mail: yeonjukim@khu.ac.kr D.-C. Yang e-mail: deokchunyang@yahoo.co.kr 123 Antonie van Leeuwenhoek (2015) 107:749–758 DOI 10.1007/s10482-014-0369-z