www.IndianJournals.com Members Copy, Not for Commercial Sale Downloaded From IP - 14.139.230.210 on dated 24-Mar-2015 BIOINFOLET 12 (1 B): 227-231,2015 IDENTIFICATION OF MOLECULAR MARKERS FOR FERTILITY RESTORATION GENES IN RICE (ORYZA SATIVA L.) Satyapal Singh, Sunil Kumar Verma, Hemant Sahu, Parmeshwar Ku. Sahu and Deepak Sharma Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya, Raipur - 492012 Chhattisgarh, lndia. ABSTRACT Present investigation was undertaken on mapping of fertility restorer gene in F3 population of IR58025AxIR72164-352-2-5-5 with the help of micro satellite markers. Among F3 population, line number 52 had high pollen and spikelet fertility, while line number 3 (0.25) showed lowest pollen and spikelet fertility, these lines may be considered as suitable for restorer and maintainer lines respectively. Four DNA markers, across 2 chromosomes (Chr1 and 10) reported to be closely linked with Rfgenes. RM490 linked with Rt; gene was located on short arm of chr1 wi th map distance of 2.8cM ; RM6100 and RM1108 were located on Chr1 0 with map distance of 1.2cM and 1.5cM, respectively from Rfgenes of the SSR markers reported in this study might be useful for the identification of CMS lines of rice through marker-assisted selection with reasonable accuracy. Key Words: Rice, Cytoplasmic Male Sterility (CMS), Wild Abortive (WA), Fertility restoration gene, SSR markers, Introduction Hybrid rice has a yield advantage of 15-20% over conventional high-yielding varieties (Virmani et al. 1994). Fertility restorer alleles (Rfs) have been evolved with cytoplasmic male sterility (CMS) duri ng plant evolution . For studying the inheritance of fertility restorers, indexes percentage of fertile pollen, bagged seed setting and opening seed-setting are used as the criteria to evaluate fertility restoration. Among these, the percentage of fertile pollens is most reliable criterion for evaluating plant fertility (Li and Yuan 1986). As CMS can be restored by nuclear genes, the CMS systems are widely used for hybrid seed production . In case of rice, most of the hybrids belong to wild abortive cytoplasmic source (Yao, et al. 1997). Three primary types of CMS in rice i.e. Wild-rice abortive (WA), BaoTai (BT) and HongLian (HL) are known to exist (Huang et at. 2003 and Ahmadikhah et at. 2003). The most popular method of marker identification is based on finding out different banding patterns in two sets of lines representing contrasting phenotypic classes, which was employed during present investigation. Material and Methods For inheritance study four crosses viz. , IR58025A x TOX 981-11-2-3, IR58025A x R1949-1196-2-1, CRMS 31A x IR72164-352- 2-5-5 and IR58025A x IR72164-352-2-5-5 were selected on the basis of pollen fertility (>80% pollen fertility) for advancement of F2 generation and were raised during Rabi2013 as per the method suggested by Virmani et al. 1994 and Virmani,2003. Cross comb ination IR58025A x IR72164-352-2-5-5 population was selected from F2 generation based on the segregation pattern, which is expected to yield dominant monogenic gene action (3:1) in F 2 . This population was used to raise F3 generation,