10.1586/ERI.12.145 1 ISSN 1478-7210 © 2012 Expert Reviews Ltd www.expert-reviews.com Review Bacterial adherence, the binding of bacteria to other cells or inanimate surfaces, is a key prop- erty that allows bacteria to survive and flour- ish. Bacteria have the ability to attach to sites in microenvironments where growth is favorable, both in ecological niches and in vivo, within a host. This property has long been known, with significant parallels established between infec- tivity and adhesiveness [1] . That adherence is a prerequisite for the subsequent development of disease symptoms for the majority of enteric, respiratory and oral infections [2] . As such, this area is increasingly becoming a source to mine for novel therapeutics in an attempt to prevent and treat bacterial infectious diseases. This will become progressively more impor- tant, as antibiotic resistance continues to increase. Among new methods to tackle infec- tious diseases, anti-adhesion therapies hold par- ticular advantages, as they do not directly cause the death of bacteria, an important selective pressure. Strains with mutations that thwart anti-adhesion mechanisms will inevitably develop; however, unlike antibiotics, which kill or stop the growth of the susceptible bacteria, nonresistant strains can continue to propagate and be transmitted to new hosts. Any wild- type strains would continue to compete with resistant strains in untreated individuals, act- ing to prevent wide propagation of resistance (FIGURE 1) [3] . Adherence and subsequent internaliza- tion confer advantages to bacteria, facilitating delivery of virulence factors and toxins, aiding acquisition of vital nutrients and evasion of both the host immune system and any antibiot- ics. Adherence confers tissue tropism, allowing specificity of the interaction between host and pathogen to ensure bacteria take advantage of the environment most appropriate to their physi- ological and metabolic requirements. By block- ing the interactions responsible for infectivity, either by prevention or dissociation, the unat- tached organisms can be eliminated by nonspe- cific cleansing mechanisms of the host, halting the development of an infection (FIGURE 2) . These clearance mechanisms vary between different regions of the body. They include the action of mucocilary removal in the airway [4] and fal- lopian tube, the flushing effect of urine in the urinary tract and bladder producing shear force, the shedding of upper epithelial cell layers and the lining of tracts and tissue with antibodies, all of which inhibit bacterial attachment. These help to prevent bacterial adherence or dislodge weakly bound bacteria for removal from the host. Alternatively, weakening the tight associa- tion allows easier elimination of the pathogen by the immune system. Anti-adhesion therapies might be deployed in several ways. Treatment might be given as prophylaxis when infection is probable, such as Daniel Cozens 1 and Robert C Read* 2 1 Department of Infection and Immunity, K Floor, Royal Hallamshire Hospital, Sheffield School of Medicine and Biomedical Science, University of Sheffield, Sheffield, S10 3JF, UK 2 Department of Clinical and Experimental Sciences, University of Southampton, Southampton General Hosptal, Tremona Rd, Southampton, SO16 6YD, UK *Author for correspondence: Tel.: +44 114 271 2237 r.c.read@soton.ac.uk Anti-adhesion therapies for bacterial infections offer an alternative to antibiotics, in which bacteria are not killed but are prevented from causing harm to a host by inhibiting adherence to host cells and tissues, a prerequisite for the majority of infectious diseases. The mechanisms of these potential therapeutic agents include inhibition of adhesins and their host receptors, vaccination with adhesins or analogs, use of probiotics and dietary supplements that interfere with receptor–adhesin interactions, subminimal inhibitory concentrations of antibiotics and manipulation of hydrophobic interactions.Once developed, these drugs will contribute to the arsenal for fighting infectious disease in the future, potentially subverting antibiotic resistance. Anti-adhesion methods as novel therapeutics for bacterial infections Expert Rev. Anti Infect. Ther. 10(12), 00–00 (2012) KEYWORDS:฀adhesins฀•฀anti-adhesion฀therapy฀•฀antibiotic฀resistance฀•฀bacterial฀adherence Author Proof