The two-toed amphiuma (Amphiuma means Garden, 1821) is a large aquatic salamander widely distributed across the southeastern United States, inhabiting a variety of lentic and lotic aquatic habitats (Johnson and Owen, 2005; Schalk and Luhring, 2010). Amphiuma means has been described both an active forager (Dundee and Rossman, 1989) as well as an ambush predator (Conant and Collins, 1998) that utilizes microhabitats that coincides with high prey availability (Schalk et al., 2010). This species has been described as an opportunistic carnivore capable of consuming a wide variety of prey (Johnson and Owen, 2005). While crayfish have been noted as being an important component of its diet, a number of vertebrates have been documented in the diet of A. means (Johnson and Owen, 2005). Known vertebrate prey species include fish, frogs, turtles, snakes, and salamanders (Johnson and Owen, 2005; Schalk et al., 2009; Mitchell, 2013). Herein we document two new vertebrate prey items for A. means. On 7 September 2014, a small A. means (SVL = 238 mm; total length = 315 mm; mouth width = 11.97 mm) was captured while electrofishing for fishes in Poplar Creek, a small forested stream in Wake County, North Carolina, USA (N35°.44.940’, W78°.28.002’). While in captivity, the A. means regurgitated two juvenile green sunfish (Lepomis cyanellus Rafinesque, 1819 [Perciformes: Centrarchidae]; first fish: standard length = 30 mm, total length = 38 mm, body depth = 10 mm; second fish: standard length = 25 mm, total length = 31 mm, body depth = 7.99 mm) and a single partially digested larval southern two-lined salamander (Eurycea cirrigera (Green, 1831) [Caudata: Plethodontidae]; SVL = 50.0 mm; head width = 7.08 mm; body depth = 7.34 mm). The A. means died a short while later and was deposited in the Herpetology Collection at the North Carolina Museum of Natural Sciences (NCSM 82406). To our knowledge, this is the first report of either of these species in the diet of A. means. While A. means has been documented as exhibiting some size preference for its prey (Taylor and Ludlam, 2013), in regards to its diet, Hamilton (1950) states that A. means eats “that which it can master”. In specimens collected from a similar part of its range in North Carolina, Brimley (1920) noted that only crayfish were found in its diet. Green sunfish and southern two- lined salamanders are common and abundant species occurring in streams in this region (Montaña, unpubl. data), their presence in the diet of A. means provides further evidence of the broad feeding habits of this generalist predator. Acknowledgements. We thank Elizabeth Hassel, Clara Navarro, and Benjamin Pluer for their assistance in the field, and Jeff Beane for his assistance at the North Carolina Museum of Natural Sciences. We also thank Brian A. Crawford for providing constructive comments on the manuscript. References Conant, R., Collins, J.T. (1998): A Field Guide to Reptiles and Amphibians of Eastern and Central North America,3rd Edition. Houghton Miffin Company, Boston. Brimley, C.S. (1920): Notes on Amphiuma and Necturus. Copeia, 1920: 5-7. Dundee, H.A., Rossman, D.A. (1989): The Amphibians and Reptiles of Louisiana, 1st Edition. Louisiana State University Press, Baton Rouge. Herpetology Notes, volume 7: 755-756 (2014) (published online on 21 December 2014) New vertebrate prey for the aquatic salamander Amphiuma means (Caudata: Amphiumidae) Carmen G. Montaña *1 , Mônica Ceneviva-Bastos 2 and Christopher M. Schalk 3 1 Department of Applied Ecology, North Carolina State University, Raleigh, NC, USA 2 UNESP – São Paulo State University, Rua Cristóvão Colombo, São José do Rio Preto, São Paulo, Brazil 3 Ecology and Evolutionary Biology Program, Department of Wildlife and Fisheries Sciences and Biodiversity Research and Teaching Collections, Texas A&M University, College Station, TX, USA * Corresponding author; e-mail: cgmontan@ncsu.edu