Journal of Environmental Science and Engineering A 2 (2013) 677-685 Formerly part of Journal of Environmental Science and Engineering, ISSN 1934-8932 Potentiality of Vermicompost Humic Acids in Banana in Vitro Micropropagation Clone: Enano Guantanamero Marcia B. Moya Fernández 1 , Luis Eduardo Cosio-Vargas 2 , Daniel Cabezas Montero 1 , Andrés Calderín García 1 , Dany Marrero López 3 and Sandra Pérez Álvarez 2 1. Deperment of Plant Protection, Agrarian University of Havana (Fructuoso Rodríguez Pérez), Mayabeque 32700, Cuba 2. INIFAP (Nacional Research Center of Forest, Agrarian and Livestock), Uruapan 60150, México 3. Havana Biofabric, Mayabeque 32700, Cuba Received: October 09, 2013 / Accepted: March 19, 2014 / Published: April 20, 2014. Abstract: The research was done at Havana Biofabric, Mayabeque Province. The vitro plants of banana (Musa sp) used came from the clone Enano Guantanamero (viand type). They come from multiplication phase in a Murashige and MS (Skoog medium). The objective of this work was to study the potentialities of HA (humic acids) for stimulation of rooting and cell division process, searching for completely synthetic hormone substitution due to its high cost. The multiplication medium contained thiamine (2 mg·L -1 ), myo-inositol (100 mg·L -1 ), sugar (30 g·L -1 ). By the use of HA, five treatments were used with total substitution of auxin and cytokinin (T1: HA (10 mg·L -1 ); (T2: HA (20 mg·L -1 ); (T3: HA (30 mg·L -1 ); (T4: HA (40 mg·L -1 ); (T5: HA (50 mg·L -1 ) and the control with 6BAP (benzil amino purine) (4 mg·L -1 ) + IBA (indol butyric acid) (0.65 mg·L -1 ). pH of the medium was adjust just before agar were add. The results showed that in the explants under HA treatments, elongation and cell multiplication were favored confirmed by explants great high, root number and length due to dry mass. The total protein content, reduced carbohydrate and peroxidases enzymatic activity were determine. The use of Vermicompost HA in the in vitro micropropagation of banana allow the elimination of rooting phase saving materials and be able to pass explants directly to Acclimatization phase. Key words: Banana, multiplication phase, HA (Humic Acids), rooting. 1. Introduction In the world, bananas are the main food for about 400 million people, with an annual production of about 92.38 million tons [1], representing the most economically important crops in tropical and subtropical countries after rice, wheat and corn [2]. The most important impacts of biotechnology application are taking place in the agricultural sector in both developed and under developing countries. Biotechnology tools have proven been very useful for crop improvement in mass propagation of higher plants [2]. The micropropagation technique is considered the most widespread and with global Corresponding author: Sandra Pérez Álvarez, Ph.D, doctor in agricultural sciences, research fields: molecular biology and biotechnology. E-mail: sandra.perez2008@gmail.com. impact inside of plant biotechnology, because it allows a fast and massive clonal multiplication. A lot of bioactive products are made in Cuba with the aim of improved economical important plant growth. Example of these products is HA (Humic Acid). The HA are the most qualified part of the organic matter, improves the physical, chemical and biological properties of the soil and influences plant growth. They can help break up compacted soils, allowing for enhanced water penetration and better root zone growth and development. When applied to sandy soils, HA adds essential organic material necessary for water retention, thus improving root growth and enhancing the sandy soil’s ability to retain and not leach out vital plant nutrients [3]. The aim of this research was to study the potentialities of HA for stimulation of rooting and cell DAVID PUBLISHING D