J. Biomedical Science and Engineering, 2014, 7, 307-315 Published Online May 2014 in SciRes. http://www.scirp.org/journal/jbise http://dx.doi.org/10.4236/jbise.2014.76033 How to cite this paper: Li, S.F., et al. (2014) Chemiluminescence Behavior of Luminol-KIO 4 -Ag Nanoparticles System and Its Analytical Applications. J. Biomedical Science and Engineering, 7, 307-315. http://dx.doi.org/10.4236/jbise.2014.76033 Chemiluminescence Behavior of Luminol-KIO 4 -Ag Nanoparticles System and Its Analytical Applications S. F. Li * , H. Y. Wang, X. Min, L. Zhang, J. Wang, J. Du, J. Q. Zhang, P. Wei, Z. Q. Wang, H. Zhang, X. W. Wu College of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Key Laboratory of Molecule-Based Materials, Anhui Normal University, Wuhu, China Email: * sfli@mail.ahnu.eu.cn Received 22 March 2014; revised 27 April 2014; accepted 5 May 2014 Copyright © 2014 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract It was found that silver nanoparticles could strongly enhance the chemiluminescence (CL) of the luminol-KIO 4 system in the presence of Co 2+ . The most intensive CL signals were obtained with silver nanoparticles in diameter of 22 nm. The studies of UV-visible spectra and CL spectra were carried out to explore the possible CL enhancement mechanism. Moreover, the influences of 17 amino acids and 25 organic compounds on the luminol-KIO 4 -Co 2+ -Ag NPs CL system were studied by a flow injection procedure, which led to an effective method to detect these compounds. Keywords Flow Injection, Luminol, Chemiluminescence, Silver Nanoparticle 1. Introduction Chemiluminescence (CL) has received many chemists’ attention due to its advantages: high sensitivity, simplici- ty, wide linear detection range and rapidity in signal detection [1]-[6]. In recent years, nanoparticles (NPs) have been widely studied for their excellent performance [7]-[11]. They display special optical, mechanical, electrical, magnetic and catalytic properties. Nanoparticles contained quantum size effect, volume effect, and surface effect that traditional solid material does not have [12]-[19]. It has been reported that metal nanoparticles have been applied to many fields, such as chemical engineering, biomedicine, environment, and electromagnetics. Using * Corresponding author.