Ž . Fitoterapia 71 2000 507510 Flavonol glycosides from leaves of Costus spiralis Alexandre da Silva Antunes, Bernadete Pereira da Silva, Jose Paz Parente  ´ Nucleo de Pesquisas de Produtos Naturais, Uni  ersidade Federal do Rio de Janeiro, Rio de ´ Janeiro, 21941-590, Brazil Received 19 January 2000; accepted 16 February 2000 Abstract A new flavonol diglycoside, 3,5-dihydroxy-7,4'-dimethoxyflavone 3-O-neohesperidoside Ž. 1 , together with four known flavonol 3-O-neohesperidosides were isolated from the leaves of Costus spiralis and their structures were elucidated by a combination of spectroscopic methods and chemical reactions.  2000 Elsevier Science B.V. All rights reserved. Keywords: Costus spiralis; 3,5-Dihydroxy-7,4'-dimethoxyflavone; 3-O-Neohesperidoside; Flavonols 1. Introduction Ž . Costus spiralis Rosc. Costaceae is used in Brazilian folk medicine in urinary   affections and for expelling urinary stones 1,2 . Pharmacological evaluation of the antiurolithiatic activity of the water extract of this plant in rats confirmed the folk  information 3 . A previous phytochemical study on C. spiralis has revealed the  occurrence of sterols and furostanol glycosides 4 . As part of our ongoing investi-   gation for the chemistry of Costus spp growing in Brazil 5-8 , here we report on the isolation and characterization from the leaves of C. spiralis of a new flavonol Ž. diglycoside, 3,5-dihydroxy-7,4'-dimethoxyflavone 3-O-neohesperidoside 1 .  Corresponding author. Tel.: 55-21-270-2683; fax: 55-21-270-2683. Ž . E-mail address: parente@nppn.ufrj.br J. Paz Parente . 0367-326X00$ - see front matter  2000 Elsevier Science B.V. All rights reserved. Ž . PII: S 0 3 6 7 - 3 2 6 X 00 00161-1