Int. J. Pharm. Sci. Rev. Res., 32(2), M ay – June 2015; Article No. 26, Pages: 161-165 ISSN 0976 – 044X International Journal of Pharmaceutical Sciences Review and Research Available online at www.globalresearchonline.net © Copyright protected. Unauthorised republication, reproduction, distribution, dissemination and copying of this document in whole or in part is strictly prohibited. © Copyright pro 161 M . Kannahi* , S. Elangeswari PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamilnadu, India. * Corresponding author’s E-mail: kannahisri79@gmail.com Accepted on: 20-04-2015; Finalized on: 31-05-2015. ABSTRACT This study was aimed to evaluate the production of Cellulase by Aspergillus niger and Trichoderma viridae were isolated and identified from soil and used for the study. Aspergillus niger and Trichoderma viridae were screened for their cellulase production ability. Cellulase production was analysed in Agro-industrial waste such as Pineapple peel, Orange peel and Banana peel. From this study, Aspergillus niger and Trichoderma viridae have high enzyme activity in Pineapple peel. The optimization study was performed on pineapple peel the optimum parameters for the isolated organism for cellulase production were studied on varying condition such as P H , temperature and substrates concentration (Carbon and Nitrogen). The maximum production of cellulase was noticed at temperature 25°C, and PH 6, in pineapple peel for Aspergillus niger than Trichoderma viridae Finally concluded that the Aspergillus niger showed highest level of cellulase production which was recommended for industrial level cellulase production. Keywords: Cellulase, Agro-industrial waste, Submerged fermentation, Aspergillus niger , Trichoderma viridae. INTRODUCTION nzymes are proteins that exhibit catalytic activity. The enzyme complex molecular structure consists of one part of protein, but it can be connected to other molecules such as carbohydrates and lipids. Enzymes are present in all living cells, which exercise the function of catalyst of the reactions that compose the anabolic and catabolic pathway of cellular metabolism. Cellulase is an important extracellular microbial enzyme, which hydrolyzes cellulose. It is also one of the most expensive sources of biomass utilized for the production of pressing in the fruit juice industry and other factories via enzyme bioconversion, which proves to have a high industrial value. A great number of microorganisms, mostly fungi are able to degrade cellulose for their growth and produced a complete set of cellulase for the hydrolysis of cellulose to soluble sugar. The agro waste such as pineapple peel, rice straw, orange peel, Banana peel etc can be used as the best substrate for bioconversion. Cellulose is commonly degraded by an enzyme called Cellulase. This enzyme is produced by several microorganisms, commonly by bacteria and fungi. Fungal genera like Trichoderma sp and Aspergillus sp and are taught to the cellulase producers and crude enzymes produced by these microorganisms are commercially available for agricultural use. Cellulases are enzymes that are capable of breaking cellulose glucosidic bonds, resulting in the release of oligosaccharides, cellobiose and glucose. These hydrolytic enzymes are used in the food, pharmaceuticals, cosmetics, detergents and textile industries. Their main applications include the bleaching of pulp in the paper industry, the production of dissolved pulp waste water treatment and recycling of paper residues. Enzym e technology is currently one of the most promising new technologies for the synthesis of valuable compounds. Industrial processes that employ enzymes in biotransformation processes have a lower environmental impact and concomitantly lower consumption of energy, since they are biodegradable and highly specific which induce undesirable effects. Cellulase production depends on the type of substrate, pretreatment and strain microorganisms used. Cellulase, it is a multi enzyme complex capable of decomposing plant substrate. One component of the cellulase enzymes system is β- Glucosidase and it work together with endoglucanase and to break down cellulose in the plant cell walls. The objective of the present study was to collect the soil sample for isolation and identification of fungi using appropriate method, to screen the cellulase producing fungal strain, to estimate the cellulase productivity using DNS method and to optimize the media formulation for the production. M ATERIALS AND M ETHODS Sample Collection Soil samples were collected from cellulosic waste dumped area at Ullikkottai, Thiruvarur District, Tamil Nadu. It was Enhanced Production of Cellulase on Different Fruit Peel Under Submerged Fermentation E Research Article