ACTA CHROMATOGRAPHICA, NO. 18, 2007 SEPARATION OF SULFUR-CONTAINING FATTY ACIDS FROM GARLIC, ALLIUM SATIVUM, USING SERIALLY COUPLED CAPILLARY COLUMNS WITH CONSECUTIVE NONPOLAR, SEMIPOLAR, AND POLAR STATIONARY PHASES  V. M. Dembitsky 1 , S. Abu-Lafi 2 , and L. O. Hanuš 1,* 1 Department of Medicinal Chemistry and Natural Products, School of Pharmacy, P.O. Box 12065, The Hebrew University of Jerusalem, Jerusalem 91120, Israel 2 Department of Chemistry and Chemical Technology, Al-Quds University, Abu-Deis, P.O. Box 20002, West Bank, Palestinian Authority SUMMARY A GC–MS method with serially coupled capillary columns contai- ning consecutive nonpolar, semipolar, and polar stationary phases has been used for determination of fatty acids in garlic (Allium sativum). Saturated (14:0, 15:0, 16:0, and 18:0), unsaturated (7-16:1, 7-18:1, 9-18:1, 9,12-18:2, 9,12,15-18:3), and unusual cyclic sulfur-containing fatty acids in garlic were identified by GC–MS. INTRODUCTION Sulfolipids were originally discovered in diatom species, in 1966, by Morris Kates [1–3]. High levels of these sulfolipids have been found in the alga Ochromonas danica (Chrysophyceae, Chrysophyta) where they con- stitute 15% of total lipids and 3% of the dry weight of heterotrophically grown, stationary-phase cells [4,5]. More recently, sulfolipids have also been identified in more than 30 species of green (Chlorophyceae), brown (Phaeophyceae), and red (Rhodophyceae) freshwater and marine macro- phytic algae [6–8] and other microalgal species [9–14]. The presence of sulfolipids in algae, diatoms, and marine invertebrates has been reported in several review articles [15–20]. –––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––  Some parts of this paper were presented at the 7th Congress of the International Society for the Study of Fatty Acids and Lipids, July 2006, Cairns, North Queensland, Australia, and at the 4th Euro Fed Lipid Congress, October 2006, Madrid, Spain - 206 -