Int. J. Pharm. Sci. Rev. Res., 29(1), November – December 2014; Article No. 13, Pages: 57-61 ISSN 0976 – 044X
International Journal of Pharmaceutical Sciences Review and Research
Available online at www.globalresearchonline.net
© Copyright protected. Unauthorised republication, reproduction, distribution, dissemination and copying of this document in whole or in part is strictly prohibited.
57
B.Janaranjani, G. Prasanna
*
, M .Chitra
PG and Research Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Thiruvarur Dt, Tamil Nadu, India.
* Corresponding author: prasannakeertana@yahoo.in
Accepted on: 27-08-2014; Finalized on: 31-10-2014.
ABSTRACT
In the present study, the methanolic extract of Drynaria quercifolia rhizome was investigated for its anti inflammatory and anti
pyretic activities on carrageenan-induced paw odema and brewer’s yeast-induced pyrexia in rats. The results revealed that the
extract at test doses of 100, 250 & 500 mg/ kg b.w produced a remarkable anti inflammatory activity at 2 ½hr with an inhibition of
paw oedema of 21%, 33% and 58% respectively, compared to the reference drug dexamethasone (200mg/ kg b w) with an inhibition
of 40%. Among the three concentrations, dose of 500 mg showed a maximum inhibition on carrageenan-induced rat paw oedema.
Anti pyretic activity was also studied on Brewer’s yeast – induced pyrexia rats. Fever was induced by injecting 10 ml/ kg (s.c) of 20%
aqueous suspension of Brewer’s yeast in normal saline and rectal temperature was recorded by clinical thermometer before and
after 12hrs of yeast administration. Drynaria quercifolia at a doses of 100, 250, 500 mg/ kg, showed significant anti-pyretic effect by
decreasing the rectal temperature. Among the three concentrations, 500mg of plant extract exhibited remarkable antipyretic
activity by decreasing the rectal temperature of rats in 1 hr (38.06
o
C), 2hr (37.33
o
C), 3hr (37.09
o
C) after treatment which was higher
than that of standard drug paracetamol (200 mg/ kg) (37.24
0
C). These findings demonstrated that Drynaria quercifolia have
remarkable anti inflammatory and anti-pyretic activities when compared with positive control and thus have great potential as a
source for natural health products.
Keywords: Anti inflammatory, Anti pyretic, Drynaria quercifolia, methanolic extract .
INTRODUCTION
nflammation is the complex biological response of
vascular tissues to harmful stimuli including
pathogens, irritants, or damaged cells. It is a protective
attempt by the organism to remove the injurious stimuli
as well as initiate the healing process for the tissue
1
. The
process of inflammation is necessary in healing of
wounds. Inflammation however, if runs unchecked, lead
to onset of diseases like vasomotor rhinnorhoea,
rheumatoid arthritis and atherosclerosis
2
.
Acute inflammation is characterised by classical signs-
edema, erythrema, pain, heat, and above all, loss of
function. The classical signs ar e triggered by the
infiltration of the tissues by serum and white blood
corpuscles (leucocytes). Chronic inflammation results in a
progressive shift in type of cells, present at site of
inflammation. It is characterized by simultaneous
destruction and healing of the injured tissue from
incidence of inflammation.
Fever or pyretic is defined as the elevation of core body
temperature above normal; in normal adults, the average
oral temperature is 36.98C (98.58F). In oncology practice,
a single temperature of more than 38.3°C (101°F) or three
readings (at least 1 hour apart) of more than 38°C (100.4°
F) are considered significant. Lower temperature
elevations in the very young or old and in patients
receiving steroids or other immunosuppressant’s are
considered abnormal
3
.
Medicinal plants are assuming greater importance in the
primary health care of individuals and communities in
many developing countries. There has been an increase of
demand in international trade because of very effective,
cheaply available, supposedly have no side effects and
used as alternative to allopathic medicines. Medicinal
plants are believed to be much safer and proved elixir in
the treatment of various ailments
4
. In recent years, the
natural antiiflammatory and antipyretics of plant origin
are being appreciated due to possible toxicity of synthetic
drugs. Keeping this view, Drynaria quercifolia w as
selected to investigate its anti inflammatory and
antipyretic activities
Drynaria quercifolia (Family: Polypodiaceae) J. smith
localy known as Gurar, is a parasitic fern
5,6
and it is
widely distributed in Bangaladesh, India, South China,
M alaisia, Australia and Thailand. It also found in low
down the mountains on tree or rocks
6,7
. Drynaria
querfcifolia usually grows in low fetile land with humid
condition. This also grows in coastal areas of India
including coastal Western Ghats of maharastra
7
.
Rhizome and roots of this plants are used as tonic in
typhoid fever and dyspepsia. Traditional use of this drug
is in diarrhea, typhoid, jaundice, fever, headache, skin
disease and syphilis. In another combination of drug,
Drynaria is used for expelling rheumatism
8
. In the
treatment of hyperthyroidism, Drynaria along with other
drugs are used. The ethnomedicinal uses of the fern
Drynaria quercifolia have been pharmacologically
confirmed by several workers such as anthelmintic
activity
9
, antibacterial and antidermatophytic activity
10
.
Antiinflammatory and Antipyretic Activities of Drynaria quercifolia Rhizome in Rats
I
Research Article