Int. J. Adv. Sci. Eng. Vol. 1 No. 3 42-46 (2015) 42 ISSN 2349 5359 Srinivasan et al International Journal of Advanced Science and Engineering www.mahendrapublications.com *Corresponding author. Email: Received: 20.12.2014 subramani895@gmail.com Accepted: 10.02.2015 Green synthesis of silver nanoparticles using Cassia auriculata flower extract and its antibacterial activity P. Srinivasan 1 , S.Sudhakar 2 , A. Sengottaiyan 1 , P. Subramani 3* , C. Sudhakar 1 , Koildhasan Manoharan 4 P. Thiyagarajan 1 1 Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal 637501, Tamil Nadu, India. 2 Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode 637 205, Tamil Nadu, India. 3 Department of Biotechnology, K.S. Rangasamy College of Arts and Science, Kalvi nagar, Tiruchengode-637 215, India 4 Department of Botany, Raja Duraisingam Government Arts College, Sivagangai 630 560, Tamil Nadu, India. INTRODUCTION Nanotechnology can be termed as the synthesis, characterization, exploration and application of nanosized (1-100nm) materials for the development of science. Nanotechnology is the study of small objects which can be used across all branches such as chemistry, biology, physics, material science and engineering [1]. Silver nanoparticles (AgNPs) have attracted and demandable research of interest in the field of nanotechnology Nanosilver has many medical applications including diagnosis, treatment, drug delivery, coating tools and medical devices. Nanosilver is used for coating medical tools and materials used in the areas of surgery, anesthesiology, cardiology and urology [2]. Eco-friendly methods for nanoparticle synthesis using plants or plant extracts [3,4], fruits [5], agricultural wastes [6], microorganisms [7], enzymes [8], have been suggested as possible alternatives to chemical and physical methods. Biological approaches to the synthesis of nanoparticles are attracting research attention [9]. Biological molecules can undergo highly controlled, hierarchical assembly [10], which makes them suitable for the development of reliable and eco friendly processes for metal nanoparticle synthesis [11]. Furthermore, these methods are cheap, rapid and efficiently produce single atoms or molecules with a wide variety of shapes (spheres, prisms, or plates) in the nano scale level Cassia auriculata is a valuable herb and widely distributed in India, Malaysia, Srilanka, Australia, Indonesia, Japan, and Vietnam, tropical Africa and the Southwestern region of the People’s Republic of China. C. auriculata is a potent anti- diabetic plant and used in folk, ayurvedic and homeopathic systems of medicine. It is also used in the treatment of asthma, eye complaints, family planning, snakebite, urinary complaints, stomach problems, piles, chronic cough, breathing troubles, colic pain, cardiopathy, constipation, dyspepsia, hemorrhoids and hepatosplenomegally. In addition, it also possesses antimicrobial, antihypercholesterolemic, anti-inflammatory and sweet suppressing activities and it also acts as feeding deterrents to caterpillar. The plant has been reported to antibacterial and microbicidal activity [12]. In the present work, an attempt has been made to synthesize silver nanoparticles using aqueous flower extract of Cassia auriculata. The characterization was done using several spectral analyses. The synthesized silver nanoparticles were evaluated for their synergistic antimicrobial activity. MATERIALS AND METHODS Collection and Preparation of the aqueous flower extract The flower of the plant namely Cassia auriculata is collected from Jalakandapuram region, Salem District, Tamil Nadu, India. The freshly collected flowers were washed with sterile double distilled water and air dried then grind with the help of mortar and pestle. The flowers were grind until it ABSTRACT: In the present study, silver nanoparticles were synthesized using flower extract of Cassia auriculata as a reducing agent by a simple and eco-friendly route. The aqueous silver ions when exposed to flower broth were reduced and resulted in green synthesis of silver nanoparticles. The reduced silver nanoparticle was characterized using different techniques such as UV-Visible spectrometer, transmission electron microscope (TEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FT-IR) analysis. Furthermore the novel approach to analyze the antimicrobial activity against human pathogenic organisms through biosynthesized silver nanoparticles. KEY WORDS: Nanoparticles, Cassia auriculata, Antibacterial activity © 2014 mahendrapublications.com, All rights reserved