ISSN 2321-807X 3609 | Page April 18, 2015 Synthesis, anti-inflammatory and molecular docking of some new 1,2,4- triazolobenzimidazol-3-yl acetone thiosemicarbazone cyclized derivatives as PLA-2 inhibitors Abdelrahman M. Mahmoud 1 , Samia G. Abdel-Moty *1 , Ola I. A. Salem 1 , Abdel-Alim M. Abdel- Alim 1 and Ahmed S. Aboraia 2 . 1 Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Assiut University, Assiut-71526, Egypt. 2 Department of Medicinal Chemistry, Faculty of Pharmacy, Assiut University, Assiut-71526, Egypt. * Corresponding author: Samia Galal Abdel-Moty E-mail: abdelmoty99@yahoo.com ABSTRACT The present work is carried out for the synthesis and evaluation of some new 1,3,4-thiadiazolines, 1,3-thiazolines and 1,3-thiazolidin-4-ones linked to 1,2,4-triazolo[1,5-a]benzimidazole as anti-inflammatory agents. Structure elucidation of these compounds was confirmed by IR, 1 H-NMR, and mass spectrometry along with elemental microanalyses. All new compounds were tested for their anti-inflammatory activity in comparison to indomethacin (INM) where some of them showed promising results comparable to INM at 4 hours interval. The most active anti-inflammatory compounds (4b, 8c and 9a) were examined on gastric mucosa and didn’t show any gastric ulcerogenic effect compared with the reference INM. Moreover, LD50 of compounds (4b and 9a) were determined in mice; they were found non toxic up to 400 mg Kg –1 (i.p.). Also, docking simulation of some compounds into PLA2 active sites was studied. Indexing terms/Keywords 1,2,4-triazolobenzimidazole, thiosemicarbazones, anti-inflammatory, ulcerogenecity, PLA2 enzyme, molecular docking. Council for Innovative Research Peer Review Research Publishing System Journal: Journal of Advances in Chemistry Vol 11, No. 5 editorjaconline@gmail.com , www.cirjac.com