International Journal of Stem Cell Research and Transplantation. 2013 © 32 Jabari F, Mohammadnejad J, Yavari K . (2013). Isolation Stem Cells from Human Dental Pulps and Differentiation Them to Adiposite and Osteoblast, Int J Stem Cell Res Transplant, 01(06), 32-36. International Journal of Stem Cell Research and Transplantation (IJST) ISSN: 2328-3548 Isolation Stem Cells From Human Dental Pulps And Differentiation Them To Adiposite And Osteoblast Research Article Jabari F 1* , Mohammadnejad J², Yavari K 3 1* Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran. 2 Professor assistance, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran 3 Professor assistance, Biotechnology lab, Nuclear Science and Technology Research institute, (NSTRI), atomic nuclear energy organization, Tehran, Iran. *Corresponding Author: Farzaneh Jabari, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran. E-mail: jabarifarzane@yahoo.com Received: October 10, 2013 Accepted: October 28, 2013 Published: October 31, 2013 Citation: Jabari F, Mohammadnejad J, Yavari K . (2013). Isolation Stem Cells from Human Dental Pulps and Differentiation Them to Adiposite and Osteoblast, Int J Stem Cell Res Transplant, 01(06), 32-36. Copyright: Jabari F © 2013. This is an open-access article distrib- uted under the terms of the Creative Commons Attribution Li- cense, which permits unrestricted use, distribution and reproduc- tion in any medium, provided the original author and source are credited. Introduction here are several differences between primary teeth and permanent teeth in terms of their evolution process, tissue architecture and their function. Stem Cells from Human Exfoliated Deciduous Teeth (SHED) have greater proliferative power in comparison to dental pulp stem cell (DPSC). Besides, these primary teeth are much smaller in size and much brighter than permanent ones. they have short Bulbaceous crown, tight Cervical canal, small rodent, narrow root, thin dentin and enamel thickness; with much wider teeth marrow which make them superior to permanent teeth. it has been shown that primary dental pulp contain stem cells which can be used as cell resource for therapeutic applica- tions like umbilical cord stem cells. To engineer tissues in vitro, the irst step is to isolate cells with the right phenotype and propagate them in suitable culturing environments. Normally, dental pulp stem cells are grown on a two-dimensional surface before being transferred to a three-dimensional scaffold construct. There was no report to our knowledge about the derivation of DPSCs from the other tooth types. The incidence of supernumerary teeth var- ies depending on the literature source. Here, we presented our re- cent success in the isolation and characterization of DPSCs from the pulp tissue of a supernumerary tooth. Materials and Methods Primary teeth falling and their replacement with permanent ones, is physiologically an active process which involves the genesis of permanent teeth and the absorption of primary teeth roots si- Abstract Objective: in this study we tried to isolated stem cells from human primary dental pulp. These cells are capable of differentiating into a variety of cell types including neural cells, adipocytes, and odontoblasts, osteoblast Background & Aim: Among different source for stem cells, less attention has been paid to the human dental pulp. Human dental pulps are the best and new source for stem cells, and have potential to different to all kinds of cells. So the aim of this study was to evaluate the pulp ability for produce stem cells. Materials and Methods: Normal exfoliated human deciduous incisors collected from6- to 12-years-old children. The pulp was sepa- rated from the crown and digested with trypsin .stem cells cultured in DMEM(Dulbecco’s Modiied Eagle’s Medium), supplemented with 20% fetal bovine serum in a 37°C incubator with 5% co 2 . The cells were differentiated into the osteoblastic and adipocytic lineages in different subcultures Results: Stem cells are able to growth and proliferate unlimitedly and differentiate to all kinds of the cells in human body. Their life time is very long and they remain their potential for long periods of time. For this reasons today many physicians and researchers consider them as a therapeutic agent to cure diseases. One of the most important advantaged of dental pulp stem cells, is that isolated from primary dental, that are thrown away usually. Various scaffolds have been used to cell culture. Stem cells were observed to grow in colony forming units, mainly composed of a ibroblast-like cell population. Conclusion: This study show that pulp contains cells with high proliferation capacity. The cells can be used for clinical application. Cells can be considered as an easy accessible source of mesenchymal stem cells for stem cell research and tissue engineering. Key Words: Dental Pulp Stem Cell; Supernumerary Tooth; Differentiation; Adiposite; Osteoblast.